Dual-Located WHIRLY1 Interacting with LHCA1 Alters Photochemical Activities of Photosystem I and Is Involved in Light Adaptation in Arabidopsis.
Dual-Located WHIRLY1 Interacting with LHCA1 Alters Photochemical Activities of Photosystem I and Is Involved in Light Adaptation in Arabidopsis.
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双位 WHIRLY1 与 LHCA1 相互作用改变光系统 I 的光化学活性并参与拟南芥的光适应
DOI：
10.3390/ijms18112352
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发表时间：
2017-11-07
影响因子：
5.6
通讯作者：
Miao Y
中科院分区：
文献类型：
--
作者：
Huang D;Lin W;Deng B;Ren Y;Miao Y
位于质体核的WHIRLY 1蛋白在调节叶片衰老中起作用，并且被认为与光合电子传递链的氧化还原态递送的活性氧的增加有关。为了明确whirly 1基因是否参与光合作用，本研究以拟南芥whirly 1基因敲除（kowhy 1）和质体定位的whirly 1基因过表达（oepWHY 1）植株为材料，检测了其光合作用的表现。与野生型相比，WHIRLY 1基因的缺失导致光系统I Y（I）的光化学量子产率和电子传递速率（ETR）的提高，以及叶绿素荧光激发能热耗散的非光化学猝灭（NPQ）的降低。进一步的分析表明，WHIRLY 1与捕光蛋白复合物I（LHCA 1）相互作用，并影响编码光系统I（PSI）和捕光复合物（LHCI）的基因的表达。此外，WHIRLY 1的缺失降低了叶绿体NAD（P）H-天冬氨酸酶样复合物（NDH）活性和NDH超复合物的积累。编码PSI-NDH复合物的几个基因在kowhy 1和whirly 1 whirly 3双突变体（ko 1/3）中也上调，但在oepWHY 1植物中稳定。然而，在强光条件下（800 μmol m−2 s−1），kowhy 1和ko 1/3植株的ETR均低于野生型，这与正常光照条件下的ETR相反。此外，在不同光照条件下，kowhy 1的几个PSI-NDH编码基因和与茉莉酸（JA）反应相关的ERF 109的表达也不同。这些结果表明，WHIRLY 1可能通过影响PSI的活性以及PSI与LHCI或NDH形成超复合物而参与了ETR的改变，并可能在质体与核之间起着通讯者的作用。
Plastid-nucleus-located WHIRLY1 protein plays a role in regulating leaf senescence and is believed to associate with the increase of reactive oxygen species delivered from redox state of the photosynthetic electron transport chain. In order to make sure whether WHIRLY1 plays a role in photosynthesis, in this study, the performances of photosynthesis were detected in Arabidopsis whirly1 knockout (kowhy1) and plastid localized WHIRLY1 overexpression (oepWHY1) plants. Loss of WHIRLY1 leads to a higher photochemical quantum yield of photosystem I Y(I) and electron transport rate (ETR) and a lower non-photochemical quenching (NPQ) involved in the thermal dissipation of excitation energy of chlorophyll fluorescence than the wild type. Further analyses showed that WHIRLY1 interacts with Light-harvesting protein complex I (LHCA1) and affects the expression of genes encoding photosystem I (PSI) and light harvest complexes (LHCI). Moreover, loss of WHIRLY1 decreases chloroplast NAD(P)H dehydrogenase-like complex (NDH) activity and the accumulation of NDH supercomplex. Several genes encoding the PSI-NDH complexes are also up-regulated in kowhy1 and the whirly1whirly3 double mutant (ko1/3) but steady in oepWHY1 plants. However, under high light conditions (800 μmol m−2 s−1), both kowhy1 and ko1/3 plants show lower ETR than wild-type which are contrary to that under normal light condition. Moreover, the expression of several PSI-NDH encoding genes and ERF109 which is related to jasmonate (JA) response varied in kowhy1 under different light conditions. These results indicate that WHIRLY1 is involved in the alteration of ETR by affecting the activities of PSI and supercomplex formation of PSI with LHCI or NDH and may acting as a communicator between the plastids and the nucleus.
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影响因子：
2.9
作者：
FLING, SP;GREGERSON, DS
通讯作者：
GREGERSON, DS
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
DOI：
10.1107/s1744309113028698
发表时间：
2013-11-01
影响因子：
0.9
作者：
Cappadocia, Laurent;Parent, Jean-Sebastien;Brisson, Normand
通讯作者：
Brisson, Normand
DOI：
10.1111/j.1432-1033.1987.tb10791.x
发表时间：
1987-03-02
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
BASSI, R;SIMPSON, D
通讯作者：
SIMPSON, D
影响因子：
64.8
作者：
Amunts, Alexey;Drory, Omri;Nelson, Nathan
通讯作者：
Nelson, Nathan