Characterization of a novel family of fibronectin-binding proteins with M23 peptidase domains from Treponema denticola.
Characterization of a novel family of fibronectin-binding proteins with M23 peptidase domains from Treponema denticola.
复制标题
DOI：
10.1111/j.2041-1014.2010.00584.x
复制
发表时间：
2010-12
影响因子：
3.7
通讯作者：
Dymock D
中科院分区：
文献类型：
--
作者：
Bamford CV;Francescutti T;Cameron CE;Jenkinson HF;Dymock D
与纤连蛋白的相互作用在一系列疾病相关细菌的毒力策略中是重要的。牙周炎相关的口腔螺旋体齿垢密螺旋体表达至少两种纤连蛋白结合蛋白，命名为Msp（主要表面蛋白）和OppA（寡肽结合蛋白同源物）。识别其他T.梅毒螺旋体纤连蛋白结合蛋白Tp 0155的氨基酸序列测定了梅毒螺旋体的纤连蛋白结合蛋白Tp 0155。齿垢基因组。七个T鉴定了编码直链淀粉蛋白的齿垢基因。除两个外，其余均在大肠杆菌中表达，纯化的重组蛋白结合纤连蛋白。使用针对Tp 0155的N-末端区域的抗体，证明T.与Tp 0155同源性最高的齿垢菌TDE 2318定位于细胞表面。如TP 0155，七个T.其中1个蛋白含有M23肽酶结构域，4个蛋白（TDE 2318、TDE 2753、TDE 1738、TDE 1297）含有1个或2个LysM结构域。M23肽酶可以降解肽聚糖，而LysM结构域识别碳水化合物聚合物。此外，TDE 1738可能作为细菌素的基础上与其他细菌溶素的同源性和存在的相邻基因编码推定的免疫因子。总的来说，这些结果表明，T。齿垢表达与细胞表面相关的纤连蛋白结合蛋白，其也可能具有细胞壁修饰或裂解功能。
Interactions with fibronectin are important in the virulence strategies of a range of disease-related bacteria. The periodontitis-associated oral spirochaete Treponema denticola expresses at least two fibronectin-binding proteins, designated Msp (major surface protein) and OppA (oligopeptide-binding protein homologue). To identify other T. denticola outer membrane fibronectin-binding proteins, the amino acid sequence of the Treponema pallidum fibronectin-binding protein Tp0155 was used to survey the T. denticola genome. Seven T. denticola genes encoding orthologous proteins were identified. All but two were expressed in Escherichia coli and purified recombinant proteins bound fibronectin. Using antibodies to the N-terminal region of Tp0155, it was demonstrated that T. denticola TDE2318, with highest homology to Tp0155, was cell surface localized. Like Tp0155, the seven T. denticola proteins contained an M23 peptidase domain and four (TDE2318, TDE2753, TDE1738, TDE1297) contained one or two LysM domains. M23 peptidases can degrade peptidoglycan whereas LysM domains recognize carbohydrate polymers. In addition, TDE1738 may act as a bacteriocin based on homology with other bacterial lysins and the presence of an adjacent gene encoding a putative immunity factor. Collectively, these results suggest that T. denticola expresses fibronectin-binding proteins associated with the cell surface that may also have cell wall modifying or lytic functions.
登录
查看更多内容
影响因子：
3.1
作者：
CHOI, BK;PASTER, BJ;GOBEL, UB
通讯作者：
GOBEL, UB
影响因子：
3.1
作者：
HAAPASALO, M;SINGH, U;UITTO, VJ
通讯作者：
UITTO, VJ
影响因子：
2.1
作者：
Fenno, JC;Wong, GWK;McBride, BC
通讯作者：
McBride, BC
DOI：
10.1099/00207713-43-2-196
发表时间：
1993-04-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
CHAN, ECS;SIBOO, R;IUGOVAZ, I
通讯作者：
IUGOVAZ, I
影响因子：
3.1
作者：
HAAPASALO, M;MULLER, KH;MCBRIDE, BC
通讯作者：
MCBRIDE, BC