Insertion and deletion mutants of vaccinia virus.
Insertion and deletion mutants of vaccinia virus.
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痘苗病毒的插入和缺失突变体。
DOI：
10.1016/0042-6822(86)90132-7
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发表时间：
1986
期刊：
影响因子：
3.7
通讯作者：
E. Paoletti
中科院分区：
文献类型：
--
作者：
M. Perkus;D. Panicali;S. Mercer;E. Paoletti
已经构建了13个有活力的牛痘病毒插入突变体。这些含有单纯疱疹病毒胸苷激酶（HSV-TK）基因编码序列的突变体是通过标记转移在体内重组产生的。利用32p -nick翻译的HSV-TK序列，利用复制滤膜技术进行原位杂交鉴定突变体，并通过反复空斑纯化获得纯培养物。其中一些插入突变体反过来被用作底物，在5 ' -溴脱氧尿苷存在下产生有活力的牛痘病毒缺失突变体。这种方法导致牛痘病毒删除了大约1.5 kb的非必需DNA的一个例子被提出。此外，对牛痘自发发生的活的缺失突变体进行了分析，该突变体缺乏大约21.4 kb的非必需DNA。
Thirteen viable insertion mutants of vaccinia virus have been constructed. These mutants, containing coding sequences of the herpes simplex virus thymidine kinase (HSV-TK) gene, were generated by marker transfer viain vivorecombination. The mutants were identified using a replica filter plating technique byin situhybridization using32P-nick translated HSV-TK sequences and obtained as pure cultures by repeated plaque purification. Some of these insertion mutants were in turn used as substrates to generate viable deletion mutants of vaccinia virus in the presence of 5′-bromodeoxyuridine. An example of this approach resulting in a vaccinia virus deleted of approximately 1.5 kb of nonessential DNA is presented. Furthermore, the analysis of spontaneously occurring viable deletion mutants of vaccinia lacking approximately 21.4 kb of nonessential DNA is described.
DOI：
10.1073/pnas.82.6.1785
发表时间：
1985-01-01
影响因子：
11.1
作者：
YEWDELL, JW;BENNINK, JR;MOSS, B
通讯作者：
MOSS, B
1型单纯疱疹病毒胸苷激酶基因的核苷酸序列。
DOI：
10.1073/pnas.78.3.1441
发表时间：
1981
影响因子：
11.1
作者：
Wagner,MJ;Sharp,JA;Summers,WC
通讯作者：
Summers,WC