Dynamics of bone healing after osteotomy with piezosurgery or conventional drilling - histomorphometrical, immunohistochemical, and molecular analysis.
Dynamics of bone healing after osteotomy with piezosurgery or conventional drilling - histomorphometrical, immunohistochemical, and molecular analysis.
复制标题
DOI：
10.1186/1479-5876-11-221
复制
发表时间：
2013-09-23
影响因子：
7.4
通讯作者：
Intini G
中科院分区：
文献类型：
--
作者：
Esteves JC;Marcantonio E Jr;de Souza Faloni AP;Rocha FR;Marcantonio RA;Wilk K;Intini G
压电手术是一种用于医学和牙科手术的截骨系统。许多研究证明了压电外科手术在切割质量、可操作性、易用性和安全性方面的临床优势。然而，很少有研究证明其在骨愈合动力学方面优于传统截骨系统。因此，本研究的目的是评估骨性手术截骨后骨愈合的动态，并将其与传统骨钻孔相关的动态进行比较。110只大鼠分为两组，每组55只。麻醉动物，手术暴露胫骨，采用压电手术（Piezo组）和常规钻孔（Drill组）制造直径2mm的缺损。分别于术后3、7、14、30、60天处死。收集骨样本并进行组织学、组织形态计量学、免疫组织化学和分子分析。在所有时间点进行组织学分析（n = 8），在术后7、14、30和60天进行组织形态计量学分析（n = 8）。免疫标记检测血管内皮生长因子（VEGF）、Caspase-3 （CAS-3）、骨保护素（OPG）、核因子κ b配体受体激活因子（RANKL）和骨钙素（OC）在第3、7和14天（n = 3）。为进行分子分析，分别于第3、7和14天处死动物，收集总RNA，采用qRT-PCR定量表达BMP信号通路、Wnt信号通路、炎症通路、成骨通路和凋亡通路相关的21个基因（n = 5）。在组织学和组织形态学上，两组的骨愈合相似，除了在手术后30天观察到的新骨量略高（p < 0.05）。免疫组织化学和qRT-PCR分析未发现所有蛋白和大部分被测基因的表达有显著差异。基于我们的研究结果，我们得出结论，在大鼠胫骨骨缺损模型中，骨愈合动力学与传统钻孔观察到的骨愈合动力学相当。
Piezosurgery is an osteotomy system used in medical and dental surgery. Many studies have proven clinical advantages of piezosurgery in terms of quality of cut, maneuverability, ease of use, and safety. However, few investigations have tested its superiority over the traditional osteotomy systems in terms of dynamics of bone healing. Therefore, the aim of this study was to evaluate the dynamics of bone healing after osteotomies with piezosurgery and to compare them with those associated to traditional bone drilling. One hundred and ten rats were divided into two groups with 55 animals each. The animals were anesthetized and the tibiae were surgically exposed to create defects 2 mm in diameter by using piezosurgery (Piezo group) and conventional drilling (Drill group). Animals were sacrificed at 3, 7, 14, 30 and 60 days post-surgery. Bone samples were collected and processed for histological, histomorphometrical, immunohistochemical, and molecular analysis. The histological analysis was performed at all time points (n = 8) whereas the histomorphometrical analysis was performed at 7, 14, 30 and 60 days post-surgery (n = 8). The immunolabeling was performed to detect Vascular Endothelial Growth Factor (VEGF), Caspase-3 (CAS-3), Osteoprotegerin (OPG), Receptor Activator of Nuclear Factor kappa-B Ligand (RANKL), and Osteocalcin (OC) at 3, 7, and 14 days (n = 3). For the molecular analysis, animals were sacrificed at 3, 7 and 14 days, total RNA was collected, and quantification of the expression of 21 genes related to BMP signaling, Wnt signaling, inflammation, osteogenenic and apoptotic pathways was performed by qRT-PCR (n = 5). Histologically and histomorphometrically, bone healing was similar in both groups with the exception of a slightly higher amount of newly formed bone observed at 30 days after piezosurgery (p < 0.05). Immunohistochemical and qRT-PCR analyses didn’t detect significant differences in expression of all the proteins and most of the genes tested. Based on the results of our study we conclude that in a rat tibial bone defect model the bone healing dynamics after piezosurgery are comparable to those observed with conventional drilling.
登录
查看更多内容
影响因子：
1.9
作者：
dos Santos, Pamela Leticia;Queiroz, Thallita Pereira;Garcia Junior, Idelmo Rangel
通讯作者：
Garcia Junior, Idelmo Rangel
影响因子：
1.9
作者：
Danza, Matteo;Guidi, Riccardo;Carinci, Francesco
通讯作者：
Carinci, Francesco
DOI：
10.1097/00001665-199001000-00011
发表时间：
1990-01-01
期刊：
The Journal of craniofacial surgery
影响因子：
--
作者：
Hollinger, J O;Kleinschmidt, J C
通讯作者：
Kleinschmidt, J C
影响因子：
1.9
作者：
Landes, Constantin A.;Stuebinger, Stefan;Sader, Robert
通讯作者：
Sader, Robert
影响因子：
15.9
作者：
Miura, M;Chen, XD;Shi, ST
通讯作者：
Shi, ST