Reverse and conventional chemical ecology approaches for the development of oviposition attractants for Culex mosquitoes.
Reverse and conventional chemical ecology approaches for the development of oviposition attractants for Culex mosquitoes.
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DOI：
10.1371/journal.pone.0003045
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发表时间：
2008-08-22
期刊：
影响因子：
3.7
通讯作者：
Furtado A
中科院分区：
文献类型：
--
作者：
Leal WS;Barbosa RM;Xu W;Ishida Y;Syed Z;Latte N;Chen AM;Morgan TI;Cornel AJ;Furtado A
合成蚊子产卵引诱剂是迫切需要的监测和控制程序的库蚊物种，这是主要的载体病原体引起的各种人类疾病，包括丝虫病，脑炎，和西尼罗河脑脊髓炎。我们采用了新的和传统的化学生态学方法来确定潜在的引诱剂，这是在现场试验中证明是有效的监测人口的Cx。p. quinquefasciatus在人类的住所。免疫组织化学研究表明，从这个物种的气味结合蛋白，CquiOBP 1，在触角上的触角，包括短，尖的触角感器类型，其中房子的嗅觉受体神经元敏感的蚊子产卵信息素（MOP），6-乙酰氧基-5-十六烷基。CquiOBP 1以单体和二聚体形式存在。单体CquiOBP 1结合MOP在pH依赖性的方式，在二级结构的变化显然与损失的结合在低pH值。信息素对映体表现出更高的亲和力比天然的立体异构体。通过使用CquiOBP 1作为结合试验和气相色谱-触角电位检测（GC-EAD）中的分子靶标，我们确定了壬醛，三甲胺（TMA）和粪臭素作为测试化合物。在巴西累西腓进行了广泛的实地评价，这是一个Cx高种群地区。p. quinquefasciatus的研究表明，TMA（0.9 µg/l）和壬醛（0.15 ng/µl）的组合在吸引力方面与目前使用的基于输液的诱饵相当，并且上级在于新开发的合成混合物消除了输液的难闻气味。
Synthetic mosquito oviposition attractants are sorely needed for surveillance and control programs for Culex species, which are major vectors of pathogens causing various human diseases, including filariasis, encephalitis, and West Nile encephalomyelitis. We employed novel and conventional chemical ecology approaches to identify potential attractants, which were demonstrated in field tests to be effective for monitoring populations of Cx. p. quinquefasciatus in human dwellings. Immunohistochemistry studies showed that an odorant-binding protein from this species, CquiOBP1, is expressed in trichoid sensilla on the antennae, including short, sharp-tipped trichoid sensilla type, which house an olfactory receptor neuron sensitive to a previously identified mosquito oviposition pheromone (MOP), 6-acetoxy-5-hexadecanolide. CquiOBP1 exists in monomeric and dimeric forms. Monomeric CquiOBP1 bound MOP in a pH-dependent manner, with a change in secondary structure apparently related to the loss of binding at low pH. The pheromone antipode showed higher affinity than the natural stereoisomer. By using both CquiOBP1 as a molecular target in binding assays and gas chromatography-electroantennographic detection (GC-EAD), we identified nonanal, trimethylamine (TMA), and skatole as test compounds. Extensive field evaluations in Recife, Brazil, a region with high populations of Cx. p. quinquefasciatus, showed that a combination of TMA (0.9 µg/l) and nonanal (0.15 ng/µl) is equivalent in attraction to the currently used infusion-based lure, and superior in that the offensive smell of infusions was eliminated in the newly developed synthetic mixture.
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DOI：
10.1016/s0006-291x(02)00185-7
发表时间：
2002-04-26
影响因子：
3.1
作者：
Ban, LP;Zhang, L;Pelosi, P
通讯作者：
Pelosi, P
DOI：
10.1073/pnas.0501447102
发表时间：
2005-04-12
影响因子：
11.1
作者：
Leal, WS;Chen, AM;Tsuruda, JM
通讯作者：
Tsuruda, JM
DOI：
10.1016/j.bbrc.2005.07.176
发表时间：
2005-10-07
影响因子：
3.1
作者：
Lautenschlager, C;Leal, WS;Clardy, J
通讯作者：
Clardy, J
影响因子：
16.2
作者：
Dobritsa, AA;van der Goes van Naters, W;Carlson, JR
通讯作者：
Carlson, JR
影响因子：
2.1
作者：
Du, YJ;Millar, JG
通讯作者：
Millar, JG