Expression of WNT1 in ameloblastoma and its significance.
Expression of WNT1 in ameloblastoma and its significance.
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WNT1在成釉细胞瘤中的表达及其意义
DOI：
10.3892/ol.2018.8820
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发表时间：
2018-08
期刊：
影响因子：
2.9
通讯作者：
Wang TF
中科院分区：
文献类型：
--
作者：
Wang GN;Zhong M;Chen Y;Ji J;Gao XQ;Wang TF
本研究旨在检测WNT 1在成釉细胞瘤（AB）中的表达。应用免疫组化方法检测80例AB、10例牙源性角化囊性瘤（KCOT）和10例正常口腔黏膜（NOM）中WNT 1的表达变化。采用Western blotting和RT-qPCR方法分别检测30例AB、5例KCOT、5例NOM和3例牙胚中WNT 1蛋白和mRNA的表达。在AB中检测到WNT 1的异位胞浆表达; 88.8%（71/80）的样本WNT 1阳性。WNT 1在AB组织中的表达量（1.74 ±0.36）显著高于NOM组织（0.57±0.05），而在KCOT组织中的表达量（0.80±0.06）无显著性差异（P>0.05）。RT-qPCR结果显示，WNT 1基因在AB组织中的表达水平是正常黏膜组织的2.43倍，是牙胚组织的1.77倍。总之，WNT 1蛋白和mRNA表达增加AB，并有异位胞浆表达。这表明WNT 1可能在AB的发生发展中起重要作用。
The present study aimed to measure the expression of WNT1 in ameloblastoma (AB). Immunohistochemistry was used to observe changes in WNT1 expression in 80 AB samples, 10 keratocystic odontogenic tumor (KCOT) samples and 10 normal oral mucosa (NOM) samples. Western blotting and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) were used to measure WNT1 protein and mRNA expression, respectively, in 30 AB samples, 5 KCOT samples, 5 NOM samples and 3 tooth germ samples. Ectopic cytoplasmic expression of WNT1 was detected in AB; 88.8% (71/80) of the samples were WNT1-positive. The western blotting results demonstrated that compared with NOM (0.57±0.05), WNT1 expression was significantly higher in AB tissue (1.74±0.36, P<0.05), whereas it was not significantly different between AB and KCOT samples (0.80±0.06, P>0.05). RT-qPCR revealed that the level of WNT1 gene expression in AB was increased 2.43-fold compared with normal mucosa, and 1.77-fold compared with tooth germ tissue. In conclusion, WNT1 protein and mRNA expression were increased in AB, and there was ectopic cytoplasmic expression. This indicates that WNT1 may serve an important role in AB occurrence and development.
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