Influence of in vitro IL-2 or IL-15 alone or in combination with Hsp-70-derived 14-mer peptide (TKD) on the expression of NK cell activatory and inhibitory receptors.
Influence of in vitro IL-2 or IL-15 alone or in combination with Hsp-70-derived 14-mer peptide (TKD) on the expression of NK cell activatory and inhibitory receptors.
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DOI：
10.1155/2013/405295
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发表时间：
2013
影响因子：
4.6
通讯作者：
Sedlackova L
中科院分区：
文献类型：
--
作者：
Hromadnikova I;Pirkova P;Sedlackova L
NK细胞代表了针对肿瘤的过继免疫治疗的潜在工具。膜结合的Hsp 70作为增强NK细胞活性的肿瘤特异性标志物。使用流式细胞术检测IL-2或IL-15单独或与Hsp 70衍生的14-mer肽（TKD）组合的体外刺激对NK活化受体表达的影响。（CD 16、NKG 2D、NKG 2C、NKp 46、NKp 44、NKp 30、KIR 2DL 4、DNAM-1和LAMP 1）和NK抑制性受体在健康个体中研究了NKG 2A、KIR 2DL 2/L3、LIR 1/ILT-2和NKR-P1 A。结果表示为受体表达细胞的百分比和CD 3 − CD 56+细胞群表达的受体量。在IL-2或IL-15单独或与TKD联合刺激后，CD 94、NKG 2D、NKp 44、NKp 30、KIR 2DL 4、DNAM-1、LAMP 1、NKG 2A和NKR-P1 A上调。KIR 2DL 2/L3仅被IL-15和IL-15/TKD上调。同时，观察到CD 94、NKp 44、NKp 30、KIR 2DL 4和LAMP 1阳性的NK细胞数量增加。IL-15和IL-15/TKD也导致KIR 2DL 2/L3和NKR-P1 A阳性细胞数量增加。仅IL-2和IL-2/TKD增加了NKG 2C和NKG 2A阳性细胞数。在CD 16、NKp 46和LIR 1/ILT-2中观察到IL-2或IL-15（含或不含TKD）对细胞表面表达的不同影响。
NK cells represent a potential tool for adoptive immunotherapy against tumors. Membrane-bound Hsp70 acts as a tumor-specific marker enhancing NK cell activity. Using flow cytometry the effect of in vitro stimulation with IL-2 or IL-15 alone or in combination with Hsp70-derived 14-mer peptide (TKD) on cell surface expression of NK activatory receptors (CD16, NKG2D, NKG2C, NKp46, NKp44, NKp30, KIR2DL4, DNAM-1, and LAMP1) and NK inhibitory receptors (NKG2A, KIR2DL2/L3, LIR1/ILT-2, and NKR-P1A) in healthy individuals was studied. Results were expressed as the percentage of receptor expressing cells and the amount of receptor expressed by CD3−CD56+ cellular population. CD94, NKG2D, NKp44, NKp30, KIR2DL4, DNAM-1, LAMP1, NKG2A, and NKR-P1A were upregulated after the stimulation with IL-2 or IL-15 alone or in combination with TKD. KIR2DL2/L3 was upregulated only by IL-15 and IL-15/TKD. Concurrently, an increase in a number of NK cells positive for CD94, NKp44, NKp30, KIR2DL4, and LAMP1 was observed. IL-15 and IL-15/TKD caused also cell number rise positive for KIR2DL2/L3 and NKR-P1A. Cell number positive for NKG2C and NKG2A was increased only by IL-2 and IL-2/TKD. The diverse effect of IL-2 or IL-15 w or w/o TKD on cell surface expression was observed in CD16, NKp46, and LIR1/ILT-2.
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影响因子：
30.5
作者：
Horng, Tiffany;Bezbradica, Jelena S.;Medzhitov, Ruslan
通讯作者：
Medzhitov, Ruslan
影响因子：
4.6
作者：
Chrul, Slawomir;Polakowska, Ewa;Bodalski, Jerzy
通讯作者：
Bodalski, Jerzy
DOI：
10.1084/jem.20021127
发表时间：
2003-01-20
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Chan HW;Kurago ZB;Stewart CA;Wilson MJ;Martin MP;Mace BE;Carrington M;Trowsdale J;Lutz CT
通讯作者：
Lutz CT
影响因子：
2.6
作者：
Decot, Veronique;Voillard, Laure;Bensoussan, Daniele
通讯作者：
Bensoussan, Daniele
影响因子：
15.9
作者：
Crane, Courtney A.;Han, Seunggu J.;Parsa, Andrew T.
通讯作者：
Parsa, Andrew T.