Differential temporal expression of milk miRNA during the lactation cycle of the marsupial tammar wallaby (Macropus eugenii).
Differential temporal expression of milk miRNA during the lactation cycle of the marsupial tammar wallaby (Macropus eugenii).
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DOI：
10.1186/1471-2164-15-1012
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发表时间：
2014-11-23
期刊：
影响因子：
4.4
通讯作者：
Lefevre C
中科院分区：
文献类型：
--
作者：
Modepalli V;Kumar A;Hinds LA;Sharp JA;Nicholas KR;Lefevre C
哺乳是哺乳动物进化的一个重要方面，沿着不同的哺乳动物谱系适应各种生殖策略。有袋类动物，如塔玛尔袋鼠，采用了一个较短的妊娠期和一个相对较长的哺乳期，新生儿是不成熟的出生和显着的发展发生在出生后的哺乳期。Tammar乳成分的持续变化可能有助于育儿袋幼崽的发育和免疫保护。在此，为了解决新鉴定的分泌性乳miRNA在这些过程中的假定贡献，对在泌乳的不同时间点从塔玛乳中收集的miRNA进行高通量测序。比较分析了哺乳期沙袋鼠乳汁和血清中miRNA的分布。结果表明，高水平的miRNA分泌在牛奶中，并允许在泌乳周期中的差异表达的牛奶miRNA的鉴定作为乳腺活动和功能的候选信号，以帮助生长和定时发育的年轻人的推定标记。对牛奶和血清中miRNA分布的比较分析表明，牛奶miRNA主要从乳腺表达，而不是从母体循环血液转移，可能通过一种新的推定的外泌体分泌途径。相比之下，与成人血液相比，在新生儿血清中可以以显著更高的水平检测到高表达的牛奶miRNAs，这表明牛奶miRNAs可能通过年轻人的肠道吸收。已经提出了miRNA在乳腺发育和分泌活动中的功能，但目前的研究结果也支持乳miRNA在调节育儿袋幼仔发育中的不同作用，揭示了哺乳动物哺乳期间母亲和幼仔之间新的潜在分子通讯。本文的在线版本（doi：10.1186/1471-2164-15-1012）包含补充材料，可供授权用户使用。
Lactation is a key aspect of mammalian evolution for adaptation of various reproductive strategies along different mammalian lineages. Marsupials, such as tammar wallaby, adopted a short gestation and a relatively long lactation cycle, the newborn is immature at birth and significant development occurs postnatally during lactation. Continuous changes of tammar milk composition may contribute to development and immune protection of pouch young. Here, in order to address the putative contribution of newly identified secretory milk miRNA in these processes, high throughput sequencing of miRNAs collected from tammar milk at different time points of lactation was conducted. A comparative analysis was performed to find distribution of miRNA in milk and blood serum of lactating wallaby. Results showed that high levels of miRNA secreted in milk and allowed the identification of differentially expressed milk miRNAs during the lactation cycle as putative markers of mammary gland activity and functional candidate signals to assist growth and timed development of the young. Comparative analysis of miRNA distribution in milk and blood serum suggests that milk miRNAs are primarily expressed from mammary gland rather than transferred from maternal circulating blood, likely through a new putative exosomal secretory pathway. In contrast, highly expressed milk miRNAs could be detected at significantly higher levels in neonate blood serum in comparison to adult blood, suggesting milk miRNAs may be absorbed through the gut of the young. The function of miRNA in mammary gland development and secretory activity has been proposed, but results from the current study also support a differential role of milk miRNA in regulation of development in the pouch young, revealing a new potential molecular communication between mother and young during mammalian lactation. The online version of this article (doi:10.1186/1471-2164-15-1012) contains supplementary material, which is available to authorized users.
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影响因子：
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作者：
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通讯作者：
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