Surface-charge-dependent cell localization and cytotoxicity of cerium oxide nanoparticles.
Surface-charge-dependent cell localization and cytotoxicity of cerium oxide nanoparticles.
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DOI：
10.1021/nn100816s
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发表时间：
2010-09-28
期刊：
影响因子：
17.1
通讯作者：
Perez JM
中科院分区：
文献类型：
--
作者：
Asati A;Santra S;Kaittanis C;Perez JM
氧化铈纳米颗粒作为抗氧化剂和辐射防护剂在癌症治疗中具有广阔的应用前景。最近，各种聚合物包覆纳米二氧化硅的制备已经被开发出来，以提高它们的水溶性，并允许这些纳米颗粒的表面功能化。然而，聚合物包被的纳米粒与细胞的相互作用，它们的摄取机制和亚细胞定位尚不清楚。在此，我们设计了具有不同表面电荷（正电荷、负电荷和中性电荷）的聚合物包被氧化铈纳米粒子，并研究了它们在正常和癌细胞系中的内化和毒性。结果表明，带正电荷或中性电荷的纳米粒进入了大多数研究细胞系，而带负电荷的纳米粒主要在癌细胞系中内化。此外，在进入细胞后，纳米粒根据纳米颗粒的表面电荷定位到不同的细胞区室（例如细胞质和溶酶体）。纳米粒的内化和亚细胞定位在纳米粒的细胞毒性中起着关键作用，当纳米粒定位在癌细胞的溶酶体中时表现出显著的毒性。相反，当它们进入细胞质或不进入细胞时，毒性最小。综上所述，这些结果表明，纳米粒子在正常细胞和癌细胞中的不同表面电荷依赖定位在纳米粒子的毒性谱中起着关键作用。
Cerium oxide nanoparticles (nanoceria) have shown great potential as antioxidant and radioprotective agents for applications in cancer therapy. Recently, various polymer-coated nanoceria preparations have been developed to improve their aqueous solubility and allow for surface functionalization of these nanoparticles. However, the interaction of polymer-coated nanoceria with cells, their uptake mechanism and subcellular localization are poorly understood. Herein, we engineered polymer-coated cerium oxide nanoparticles with different surface charge (positive, negative and neutral) and studied their internalization and toxicity in normal and cancer cell lines. Results showed that nanoceria with a positive or neutral charge enters most of the cell lines studied, while nanoceria with a negative charge internalizes mostly in the cancer cell lines. Moreover, upon entry into the cells, nanoceria is localized to different cell compartments (e.g. cytoplasm and lysosomes) depending on the nanoparticle's surface charge. The internalization and subcellular localization of nanoceria plays a key role in the nanoparticles’ cytotoxicity profile, exhibiting significant toxicity when they localize in the lysosomes of the cancer cells. In contrast, minimal toxicity is observed when they localize into the cytoplasm or do not enter the cells. Taken together, these results indicate that the differential surface-charge-dependent localization of nanoceria in normal and cancer cells plays a critical role in the nanoparticles’ toxicity profile.
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影响因子：
1.5
作者：
Chen, HHC;Yu, C;Chiang, LY
通讯作者：
Chiang, LY
影响因子：
17.1
作者：
George, Saji;Pokhrel, Suman;Xia, Tian;Gilbert, Benjamin;Ji, Zhaoxia;Schowalter, Marco;Rosenauer, Andreas;Damoiseaux, Robert;Bradley, Kenneth A.;Maedler, Lutz;Nel, Andre E.
通讯作者：
Nel, Andre E.
影响因子：
158.5
作者：
Harisinghani, MG;Barentsz, J;Weissleder, R
通讯作者：
Weissleder, R
DOI：
10.1080/15363839708013327
发表时间：
1997-01-01
期刊：
FULLERENE SCIENCE AND TECHNOLOGY
影响因子：
--
作者：
Chen, HHC;Yu, C;Chiang, LY
通讯作者：
Chiang, LY
影响因子：
10.8
作者：
Niu, Jianli;Azfer, Asim;Kolattukudy, Pappachan E.
通讯作者：
Kolattukudy, Pappachan E.