The chemical signatures underlying host plant discrimination by aphids.
The chemical signatures underlying host plant discrimination by aphids.
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DOI：
10.1038/s41598-017-07729-0
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发表时间：
2017-08-17
影响因子：
4.6
通讯作者：
Butlin RK
中科院分区：
文献类型：
--
作者：
Hopkins DP;Cameron DD;Butlin RK
植食性昆虫的多样性很大程度上归因于宿主植物之间的物种形成。这些变化是由昆虫和植物代谢物之间的密切相互作用介导的。然而，在理解宿主偏好背后的化学特征方面进展有限。我们使用豌豆蚜 (Acyrthosiphon pisum) 来解决这个问题。豌豆蚜的寄主相关种族以特定种族的方式区分植物物种。我们将多种植物物种的代谢组学分析与两个豌豆品种的行为测试相结合，以确定解释接受或歧视差异的代谢物。使用串联质谱法鉴定了候选化合物。我们的结果揭示了少数化合物，这些化合物解释了植物对豌豆小种的不同可接受性的大部分变化。其中两种被鉴定为 L-苯丙氨酸和 L-酪氨酸，但代谢相关的化合物可能直接影响昆虫的行为。与不同的可接受性有关的化合物与与植物对蚜虫的一般可接受性相关的组无关，无论寄主种族如何。对常见代谢物反应的微小变化可能是宿主转移的基础。这项研究为理解昆虫宿主歧视和宿主转移的机制基础开辟了新的机会。
The diversity of phytophagous insects is largely attributable to speciation involving shifts between host plants. These shifts are mediated by the close interaction between insects and plant metabolites. However, there has been limited progress in understanding the chemical signatures that underlie host preferences. We use the pea aphid (Acyrthosiphon pisum) to address this problem. Host-associated races of pea aphid discriminate between plant species in race-specific ways. We combined metabolomic profiling of multiple plant species with behavioural tests on two A. pisum races, to identify metabolites that explain variation in either acceptance or discrimination. Candidate compounds were identified using tandem mass spectrometry. Our results reveal a small number of compounds that explain a large proportion of variation in the differential acceptability of plants to A. pisum races. Two of these were identified as L-phenylalanine and L-tyrosine but it may be that metabolically-related compounds directly influence insect behaviour. The compounds implicated in differential acceptability were not related to the set correlated with general acceptability of plants to aphids, regardless of host race. Small changes in response to common metabolites may underlie host shifts. This study opens new opportunities for understanding the mechanistic basis of host discrimination and host shifts in insects.
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影响因子：
10.7
作者：
Duvaux L;Geissmann Q;Gharbi K;Zhou JJ;Ferrari J;Smadja CM;Butlin RK
通讯作者：
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影响因子：
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