Characterization of the myometrial transcriptome and biological pathways of spontaneous human labor at term.
Characterization of the myometrial transcriptome and biological pathways of spontaneous human labor at term.
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DOI：
10.1515/jpm.2010.097
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发表时间：
2010-11
影响因子：
2.4
通讯作者：
Kim CJ
中科院分区：
文献类型：
--
作者：
Mittal P;Romero R;Tarca AL;Gonzalez J;Draghici S;Xu Y;Dong Z;Nhan-Chang CL;Chaiworapongsa T;Lye S;Kusanovic JP;Lipovich L;Mazaki-Tovi S;Hassan SS;Mesiano S;Kim CJ
描述足月自然分娩过程中人子宫肌层的转录组特征。子宫肌层取自分娩妇女（n=19）和未分娩妇女（n=20）。使用Illumina® HumanHT-12微阵列。应用中度t检验和p值的错误发现率调整。在一组单独的样品中对一组选择的差异表达基因进行qRT-PCR。利用ELISA和蛋白质印迹来确认第三样品组中的差异蛋白质产生。1)471个基因差异表达; 2）基因本体论分析表明富集了103个生物学过程和18个分子功能，包括：a）炎症反应; B）细胞因子活性;和c）趋化因子活性; 3）使用信号通路影响分析的系统生物学通路分析表明6个重要通路：a）丝氨酸-细胞因子受体相互作用; B）Jak-Stat信号通路;和c）补体和凝血级联; d）NOD样受体信号传导途径; e）系统性红斑狼疮;和f）趋化因子信号传导途径; 3）qRT-PCR证实了拟南芥素-内过氧化物合酶-2的过表达（PTGS 2/COX 2）、肝素结合EGF样生长因子（HBEGF）、趋化因子C-C基序配体2（CCL 2/MCP 1），白细胞免疫球蛋白样受体，亚家族A成员5（LILRA 5/LIR 9）、IL-8、IL-6、趋化因子C-X-C基序配体6（CXCL 6/GCP 2），B细胞中κ轻链基因增强子的核因子抑制剂ζ（NFKBIZ），细胞因子信号转导抑制因子3（SOCS 3）和FK 506结合蛋白5（FKBP 5）和乙醛脱氢酶2（ALDH 2）在分娩中的表达降低; 4）足月分娩组IL-6、CXCL 6、CCL 2和SOCS 3蛋白表达均显著高于足月未分娩组。足月自然分娩妇女子宫肌层的特征是与炎症反应和白细胞趋化性过度表达一致的刻板基因表达模式。使用一组独立的样品，用qRT-PCR证实用微阵列鉴定的差异基因表达。这项研究代表了一个公正的描述自然分娩的生物过程中涉及的基础上，在长期的转录组学。
To characterize the transcriptome of human myometrium during spontaneous labor at term. Myometrium was obtained from women with (n=19) and without labor (n=20). Illumina® HumanHT-12 microarrays were utilized. Moderated t-tests and False Discovery Rate adjustment of p-values were applied. qRT-PCR was performed for a select set of differentially expressed genes in a separate set of samples. ELISA and Western Blot were utilized to confirm differential protein production in a third sample set. 1) 471 genes were differentially expressed; 2) Gene Ontology analysis indicated enrichment of 103 biological processes and 18 molecular functions including: a) inflammatory response; b) cytokine activity; and c) chemokine activity; 3) systems biology pathway analysis using Signaling Pathway Impact Analysis indicated 6 significant pathways: a) cytokine-cytokine receptor interaction; b) Jak-Stat signaling; and c) complement and coagulation cascades; d) NOD-like receptor signaling pathway; e) Systemic Lupus Erythematosus; and f) Chemokine signaling pathway; 3) qRT-PCR confirmed over-expression of prostaglandin-endoperoxide synthase-2 (PTGS2/COX2), heparin binding EGF-like growth factor (HBEGF), chemokine C-C motif ligand 2 (CCL2/MCP1), leukocyte immunoglobulin-like receptor, subfamily A member 5 (LILRA5/LIR9), IL-8, IL-6, chemokine C-X-C motif ligand 6 (CXCL6/GCP2), nuclear factor of kappa light chain gene enhancer in B-cells inhibitor zeta (NFKBIZ), suppressor of cytokine signaling 3 (SOCS3) and decreased expression of FK506 binding-protein 5 (FKBP5) and aldehyde dehydrogenase (ALDH2) in labor; 4) IL-6, CXCL6, CCL2 and SOCS3 protein expression was significantly higher in the term labor group compared to the term not in labor group. Myometrium of women in spontaneous labor at term is characterized by a stereotypic gene expression pattern consistent with over-expression of the inflammatory response and leukocyte chemotaxis. Differential gene expression identified with microarray was confirmed with qRT-PCR using an independent set of samples. This study represents an unbiased description of the biological processes involved in spontaneous labor at term based on transcriptomics.
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影响因子：
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作者：
Bethin, KE;Nagai, Y;Muglia, LJ
通讯作者：
Muglia, LJ
影响因子：
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Mitchell, Bryan F.
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影响因子：
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通讯作者：
Gibb, W