Anticancer Activity of Binary Toxins from Lysinibacillus sphaericus IAB872 against Human Lung Cancer Cell Line A549
Anticancer Activity of Binary Toxins from Lysinibacillus sphaericus IAB872 against Human Lung Cancer Cell Line A549
复制标题
球形赖氨酸芽孢杆菌 IAB872 二元毒素对人肺癌细胞系 A549 的抗癌活性
DOI：
10.1177/1934578x1400900131
复制
发表时间：
2014-01
影响因子：
1.8
通讯作者：
Bei Han
中科院分区：
文献类型：
--
作者：
Cuicui Liu;Ruijuan Zhang;Jianwei He;Bei Han
采用MTT法检测球形赖氨酸芽孢杆菌IAB 872产生的双毒蛋白（Bin）对人肺、肝、胃和宫颈癌细胞株的增殖抑制作用。采用MTT法、Western blotting、免疫细胞化学染色、流式细胞术和创伤愈合实验检测Bin蛋白对A549细胞增殖、凋亡、细胞周期、迁移和侵袭能力的影响。结果显示，Bin蛋白在体外抑制一系列人类癌细胞的增殖。Bin的抗增殖作用与Bax/bcl-2比值升高、CyclinB 1表达上调、Cdc 25 c表达下调导致细胞凋亡有关，其抗增殖作用与细胞周期阻滞在G2/M期有关。Bin蛋白可促进A549细胞凋亡，抑制A549细胞的运动和侵袭能力。Bin蛋白的抗增殖作用与诱导细胞凋亡和细胞周期紊乱有关。这些结果表明Bin蛋白具有通过诱导人肿瘤细胞凋亡而开发为化疗剂的潜力。
The inhibitory effect of binary toxic (Bin) protein produced by Lysinibacillus sphaericus IAB872 on cell proliferation of human lung, liver, stomach and cervical tumor cell lines was assessed using MTT assay. The effect of Bin protein on A549 cell proliferation, apoptosis, cell cycle, migration and invasion were examined by MTT assay, Western blotting, Immunocytochemical staining, flow cytometry assay and wound-healing assay. Results showed that Bin protein inhibits proliferation of a range of human cancer cells in vitro. The anti-proliferative effect of Bin is associated with cell apoptosis as a result of an increased ratio of cellular Bax/bcl-2, up-regulated CyclinB1and down-regulated Cdc25c expression, and its anti-proliferative action was associated with cell cycle arrest in the G2/M-phase. Bin protein could promote apoptosis and inhibit motility and invasion of A549 cancer cells. The anti-proliferative effect of Bin protein was associated with the induction of apoptotic cell death and cell cycle disruption. These results show that Bin protein has the potential to be developed as a chemotherapeutic agent by induction of human tumor cell apoptosis.
登录
查看更多内容
影响因子：
4.8
作者：
McNutt, Markey C.;Lagace, Thomas A.;Horton, Jay D.
通讯作者：
Horton, Jay D.
影响因子：
2
作者：
M. Ohba;E. Mizuki;Akiko Uemori
通讯作者：
M. Ohba;E. Mizuki;Akiko Uemori
影响因子：
2.1
作者：
M. El‐Bendary;F. Priest;J. Charles;W. Mitchell
通讯作者：
M. El‐Bendary;F. Priest;J. Charles;W. Mitchell
影响因子：
2.7
作者：
Grisolia, Cesar Koppe;Oliveira-Filho, Eduardo Cyrino;Monnerat, Rose Gomes
通讯作者：
Monnerat, Rose Gomes
影响因子：
4
作者：
Kim, HS;Yamashita, S;Ohba, M
通讯作者：
Ohba, M