Modelling at-level allodynia after mid-thoracic contusion in the rat.
Modelling at-level allodynia after mid-thoracic contusion in the rat.
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DOI：
10.1002/ejp.1711
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发表时间：
2021-04
期刊：
影响因子：
--
通讯作者：
Moxon KA
中科院分区：
文献类型：
--
作者：
Blumenthal GH;Nandakumar B;Schnider AK;Detloff MR;Ricard J;Bethea JR;Moxon KA
大鼠胸中部挫伤模型已被用于研究水平触觉异常性疼痛，这是脊髓损伤（SCI）后出现的一种常见类型的疼痛。该模型的一个重要优点是，并非所有动物都会发生超敏反应。因此，它可以用来检查与疼痛样行为的发展严格相关的机制，而不是与损伤本身相关的机制。然而，如何将发生超敏反应的动物与未发生超敏反应的动物区分开来尚不清楚。本研究的目的是确定超敏反应和痉挛的发生位置，并使用此信息确定将发生超敏反应的动物与未发生超敏反应的动物分开的指标，以研究其行为差异。为了实现这些目标，使用网格来定位相对于胸部皮节的背侧躯干上的超敏反应，并记录对触觉刺激的脊髓上反应。这些脊髓上反应用于开发超敏反应评分，以分离对非疼痛刺激产生超敏反应或疼痛样反应的动物。与人类相似，超敏反应的发生可能伴随痉挛或反射亢进的发生。此外，由该模型产生的超敏反应表型（处于、高于或低于水平）的时程和患病率与在SCI患者中观察到的相似。然而，脊髓中备用脊髓物质的量并不能解释超敏反应的发生，如以前报道的那样。这种方法可用于研究超敏反应发展的机制，与单独损伤相关的机制分开。
The rat mid-thoracic contusion model has been used to study at-level tactile allodynia, a common type of pain that develops after spinal cord injury (SCI). An important advantage of this model is that not all animals develop hypersensitivity. Therefore, it can be used to examine mechanisms that are strictly related to the development of pain-like behaviour separately from mechanisms related to the injury itself. However, how to separate animals that develop hypersensitivity from those that do not is unclear. The aims of the current study were to identify where hypersensitivity and spasticity develop and use this information to identify metrics to separate animals that develop hypersensitivity from those that do not to study differences in their behaviour. To accomplish these aims, a grid was used to localize hypersensitivity on the dorsal trunk relative to thoracic dermatomes and supraspinal responses to tactile stimulation were tallied. These supraspinal responses were used to develop a hypersensitivity score to separate animals that develop hypersensitivity, or pain-like response to nonpainful stimuli. Similar to humans, the development of hypersensitivity could occur with the development of spasticity or hyperreflexia. Moreover, the time course and prevalence of hypersensitivity phenotypes (at-, above-, or below level) produced by this model were similar to that observed in humans with SCI. However, the amount of spared spinal matter in the cord did not explain the development of hypersensitivity, as previously reported. This approach can be used to study the mechanisms underlying the development of hypersensitivity separately from mechanisms related to injury alone.
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影响因子：
4.7
作者：
Gwak, Young S.;Hulsebosch, Claire E.
通讯作者：
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DOI：
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发表时间：
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期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
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