Analysis on blast fungus-responsive characters of a flavonoid phytoalexin sakuranetin; accumulation in infected rice leaves, antifungal activity and detoxification by fungus.
Analysis on blast fungus-responsive characters of a flavonoid phytoalexin sakuranetin; accumulation in infected rice leaves, antifungal activity and detoxification by fungus.
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DOI：
10.3390/molecules190811404
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发表时间：
2014-08-04
期刊：
影响因子：
--
通讯作者：
Ohashi Y
中科院分区：
文献类型：
--
作者：
Hasegawa M;Mitsuhara I;Seo S;Okada K;Yamane H;Iwai T;Ohashi Y
为了解水稻黄酮类抗瘟素（PA）樱草素对稻瘟病的抗性作用，研究了其对真菌的反应特性。在孢子悬浮液接种后（dpi）的3天内，抗性系中的水稻幼叶表现出过敏反应（HR），并且在3dpi检测到樱花毒素的增加，在4dpi增加到4倍。在敏感线，增加樱花，检测到在4 dpi，但不是在3 dpi，其中一个大的真菌质量已经积累了没有HR。诱导表达的PA生物合成基因OsNOMT的柚皮素7-O-甲基转移酶被发现在两个品种的樱花积累之前。在相似的实验条件下，樱花醇的体外和体内抗真菌活性明显高于主要的水稻二萜PA momilactone A。在固体和液体菌丝体培养物中均检测到樱花内酯的降解和解毒，且降解和解毒的过程比豆蔻内酯A慢。在HR病斑处估计的局部樱花素浓度被认为是有效的真菌限制，而在感病水稻的扩大病斑处的估计的局部樱花素浓度是不够的。这些结果表明，樱花可能参与稻瘟病抗性及其与稻瘟病真菌的特定关系。
To understand the role of the rice flavonoid phytoalexin (PA) sakuranetin for blast resistance, the fungus-responsive characteristics were studied. Young rice leaves in a resistant line exhibited hypersensitive reaction (HR) within 3 days post inoculation (dpi) of a spore suspension, and an increase in sakuranetin was detected at 3 dpi, increasing to 4-fold at 4 dpi. In the susceptible line, increased sakuranetin was detected at 4 dpi, but not at 3 dpi, by which a large fungus mass has accumulated without HR. Induced expression of a PA biosynthesis gene OsNOMT for naringenin 7-O-methyltransferase was found before accumulation of sakuranetin in both cultivars. The antifungal activity of sakuranetin was considerably higher than that of the major rice diterpenoid PA momilactone A in vitro and in vivo under similar experimental conditions. The decrease and detoxification of sakuranetin were detected in both solid and liquid mycelium cultures, and they took place slower than those of momilactone A. Estimated local concentration of sakuranetin at HR lesions was thought to be effective for fungus restriction, while that at enlarged lesions in susceptible rice was insufficient. These results indicate possible involvement of sakuranetin in blast resistance and its specific relation to blast fungus.
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影响因子：
3.2
作者：
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通讯作者：
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影响因子：
3.5
作者：
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7.4
作者：
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5.6
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影响因子：
1.6
作者：
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