Focal cerebral ischemia induces a multilineage cytogenic response from adult subventricular zone that is predominantly gliogenic.
Focal cerebral ischemia induces a multilineage cytogenic response from adult subventricular zone that is predominantly gliogenic.
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DOI：
10.1002/glia.21033
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发表时间：
2010-10
期刊：
影响因子：
6.2
通讯作者：
Cunningham, Lee Anna
中科院分区：
文献类型：
--
作者：
Li, Lu;Harms, Kate M.;Ventura, P. Britten;Lagace, Diane C.;Eisch, Amelia J.;Cunningham, Lee Anna
The purpose of this study was to ascertain the relative contribution of neural stem/progenitor cells (NSPCs) of the subventricular zone (SVZ) to lineages that repopulate the injured striatum following focal ischemia. We utilized a tamoxifen-inducible Cre/loxP system under control of the nestin promoter, which provides permanent YFP labeling of multipotent nestin+ SVZ-NSPCs prior to ischemic injury and continued YFP expression in all subsequent progeny following stroke. YFP reporter expression was induced in adult male nestin-CreERT2:R26R-YFP mice by tamoxifen administration (180 mg/kg, daily for 5 days). Fourteen days later, mice were subjected to 60 minute transient middle cerebral artery occlusion (MCAO) and sacrificed at 2 days, 2 weeks or 6 weeks post-MCAO for phenotypic fate mapping of YFP+ cells using lineage-specific markers. Migration of YFP+ cells from SVZ into the injured striatal parenchyma was apparent at 2 and 6 weeks, but not 2 days, post-MCAO. At 2 weeks post-MCAO, the average percent distribution of YFP+ cells within the injured striatal parenchyma was as follows: 10% Dcx+ neuroblasts, 15–20% oligodendrocyte progenitors, 59% GFAP+ astrocytes, and only rare NeuN+ postmitotic neurons. A similar phenotypic distribution was observed at 6 weeks, except for an increased average percentage of YFP+ cells that expressed Dcx+ (20%) or NeuN (5%). YFP+ cells did not express endothelial markers, but displayed unique anatomical relationships with striatal vasculature. These results indicate that nestin+ NSPCs within the SVZ mount a multilineage response to stroke that includes a gliogenic component more predominant than previously appreciated.
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DOI：
10.1634/stemcells.2008-0519
发表时间：
2008-12
期刊：
Stem cells (Dayton, Ohio)
影响因子：
--
作者：
Barkho BZ;Munoz AE;Li X;Li L;Cunningham LA;Zhao X
通讯作者：
Zhao X
影响因子：
5.3
作者：
Ohab, John J.;Fleming, Sheila;Carmichael, S. Thomas
通讯作者：
Carmichael, S. Thomas
影响因子：
5.3
作者：
Lee, SR;Kim, HY;Lo, EH
通讯作者：
Lo, EH
DOI：
10.1073/pnas.0603512103
发表时间：
2006-08-29
影响因子：
11.1
作者：
Jin, Kunlin;Wang, Xiaomei;Greenberg, David A.
通讯作者：
Greenberg, David A.
脊髓损伤揭示了室室室细胞的多链分化。
影响因子：
9.8
作者：
Meletis, Konstantinos;Barnabe-Heider, Fanie;Carlen, Marie;Evergren, Emma;Tomilin, Nikolay;Shupliakov, Oleg;Frisen, Jonas
通讯作者：
Frisen, Jonas