Electroacupuncture modulates the intestinal microecology to improve intestinal motility in spinal cord injury rats.
Electroacupuncture modulates the intestinal microecology to improve intestinal motility in spinal cord injury rats.
复制标题
电针调节肠道微生态改善脊髓损伤大鼠肠道蠕动
DOI：
10.1111/1751-7915.13968
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发表时间：
2022-03
影响因子：
5.7
通讯作者：
Zhu Y
中科院分区：
文献类型：
--
作者：
Cheng J;Li W;Wang Y;Cao Q;Ni Y;Zhang W;Guo J;Chen B;Zang Y;Zhu Y
脊髓损伤（SCI）是一种涉及胃肠道疾病的疾病。电针（EA）和5‐羟色胺（5‐HT）系统对脊髓损伤的潜在保护作用机制尚不清楚。我们研究了EA是否能改善脊髓损伤患者的肠道微生物失调并调节5‐HT系统。应用16S rDNA基因测序研究大鼠肠道微生物组的变化。检测粪便代谢物和5‐HT系统的表达。大肠杆菌和粪便菌群移植（FMT）治疗促进了脊髓损伤大鼠肠传递功能的恢复和结肠形态的恢复。SCI大鼠肠道菌群组成，包括变形菌门、梭状芽胞杆菌纲、拟杆菌目和Dorea属的数量被扩增，EA和FMT显著重塑了肠道菌群。脊髓损伤导致大鼠代谢紊乱，与脊髓损伤大鼠相比，EA和FMT组儿茶素含量增加。SCI抑制结肠中5‐HT系统的表达，EA和FMT治疗显著逆转了这一现象。因此，EA可能通过调节微生物群和代谢物以及调节5‐HT系统来改善SCI。我们的研究从微生物群和5‐HT调控的角度为脊髓损伤的发病机制和治疗提供了新的见解。EA可改善脊髓损伤大鼠的肠道功能和病理。EA和FMT治疗改变了脊髓损伤大鼠肠道菌群的失调。EA和FMT处理会影响脊髓损伤大鼠体内某些细菌的丰度。
Spinal cord injury (SCI) is a disease involving gastrointestinal disorders. The underlying mechanisms of the potential protective effects of electroacupuncture (EA) and 5‐hydroxytryptamine (5‐HT) system on SCI remain unknown. We investigated whether EA improves gut microbial dysbiosis in SCI and regulates the 5‐HT system. 16S rDNA gene sequencing was applied to investigate alterations in the gut microbiome of the rats. Faecal metabolites and the expression of the 5‐HT system were detected. EA and faecal microbiota transplantation (FMT) treatment facilitated intestinal transmission functional recovery and restored the colon morphology of SCI rats. The composition of the intestinal microbiota, including numbers of phylum Proteobacteria, class Clostridia, order Bacteroidales, and genus Dorea, were amplified in SCI rats, and EA and FMT significantly reshaped the intestinal microbiota. SCI resulted in disturbed metabolic conditions in rats, and the EA and FMT group showed increased amounts of catechin compared with SCI rats. SCI inhibited 5‐HT system expression in the colon, which was significantly reversed by EA and FMT treatment. Therefore, EA may ameliorate SCI by modulating microbiota and metabolites and regulate the 5‐HT system. Our study provides new insights into the pathogenesis and therapy of SCI from the perspective of microbiota and 5‐HT regulation. EA ameliorated the intestinal function and pathology of SCI rats. EA and FMT treatment alters the dysbiosis of the gut microbiome of SCI rats. EA and FMT treatment affects the abundance of certain bacteria in SCI rat.
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影响因子：
11
作者：
Lopes Lda S;Marques RB;Fernandes HB;Pereira Sda S;Ayres MC;Chaves MH;Almeida FR
通讯作者：
Almeida FR
影响因子：
3.6
作者：
Khalatbary, Ali Reza;Khademi, Emad
通讯作者：
Khademi, Emad
影响因子：
3.5
作者：
Hussain, Z.;Lee, Y. J.;Park, H.
通讯作者：
Park, H.
影响因子：
1.7
作者：
Aura, Anna-Marja;Mattila, Ismo;Oresic, Matej
通讯作者：
Oresic, Matej
影响因子：
3.7
作者：
Gungor B;Adiguzel E;Gursel I;Yilmaz B;Gursel M
通讯作者：
Gursel M