Diurnal changes in pathogenic and indicator virus concentrations in wastewater.
Diurnal changes in pathogenic and indicator virus concentrations in wastewater.
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DOI：
10.1007/s11356-023-30381-3
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发表时间：
2023-12
影响因子：
5.8
通讯作者：
Jones, Davey L.
中科院分区：
文献类型：
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作者：
Farkas, Kata;Pantea, Igor;Woodhall, Nick;Williams, Denis;Lambert-Slosarska, Kathryn;Williams, Rachel C.;Grimsley, Jasmine M. S.;Singer, Andrew C.;Jones, Davey L.
基于水的流行病学（WBE）通常用于监测SARS-CoV-2疫情。由于采样时间和方法（即抓斗与复合）可能会有所不同，应更好地了解污水中病毒浓度的日变化。在这项研究中，我们收集了未经处理的废水样品，每小时4天，在威尔士的两个污水处理厂，以建立病毒浓度和水的物理化学性质的昼夜模式。同时，我们还试验了三种吸收材料作为被动样品，作为收集复合样品的简单且具有成本效益的替代方案。96%的液体样本（n = 74）和88%的被动采样者（n = 59）对SARS-CoV-2呈阳性，而87%和97%的液体和被动样本分别对粪便指示病毒培养呈阳性。我们发现目标病毒、铵和正磷酸盐的浓度没有显著的每日变化，pH值和电导率水平也很稳定。部分理化性质与病毒浓度呈弱正相关。更多的变化，观察到从流入液流中的样品，而不是那些从流入槽。在作为被动样本试验的吸收性材料中，我们发现卫生棉条提供的病毒回收率高于负电性滤纸和棉纱拭子。对于所有供试材料，病毒回收率取决于病毒类型。我们的研究结果表明，抓斗样品可以提供代表性的替代24小时复合样品，如果从进水池，从而降低WBE方案的采样成本。卫生棉条也是具有成本效益的采样的可行替代品;但是，在使用前应优化病毒回收。在线版本包含补充材料，可通过10.1007/s11356-023-30381-3获得。
Wastewater-based epidemiology (WBE) has been commonly used for monitoring SARS-CoV-2 outbreaks. As sampling times and methods (i.e. grab vs composite) may vary, diurnal changes of viral concentrations in sewage should be better understood. In this study, we collected untreated wastewater samples hourly for 4 days at two wastewater treatment plants in Wales to establish diurnal patterns in virus concentrations and the physico-chemical properties of the water. Simultaneously, we also trialled three absorbent materials as passive samples as a simple and cost-efficient alternative for the collection of composite samples. Ninety-six percent of all liquid samples (n = 74) and 88% of the passive samplers (n = 59) were positive for SARS-CoV-2, whereas 87% and 97% of the liquid and passive samples were positive for the faecal indicator virus crAssphage, respectively. We found no significant daily variations in the concentration of the target viruses, ammonium and orthophosphate, and the pH and electrical conductivity levels were also stable. Weak positive correlations were found between some physico-chemical properties and viral concentrations. More variation was observed in samples taken from the influent stream as opposed to those taken from the influent tank. Of the absorbent materials trialled as passive samples, we found that tampons provided higher viral recoveries than electronegative filter paper and cotton gauze swabs. For all materials tested, viral recovery was dependent on the virus type. Our results indicate that grab samples may provide representative alternatives to 24-h composite samples if taken from the influent tank, hence reducing the costs of sampling for WBE programmes. Tampons are also viable alternatives for cost-efficient sampling; however, viral recovery should be optimised prior to use. The online version contains supplementary material available at 10.1007/s11356-023-30381-3.
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DOI：
10.1016/j.coesh.2020.09.003
发表时间：
2020-09-30
影响因子：
--
作者：
Ahmed W;Bivins A;Bertsch PM;Bibby K;Choi PM;Farkas K;Gyawali P;Hamilton KA;Haramoto E;Kitajima M;Simpson SL;Tandukar S;Thomas K;Mueller JF
通讯作者：
Mueller JF
影响因子：
2.4
作者：
Farkas K;Hillary LS;Thorpe J;Walker DI;Lowther JA;McDonald JE;Malham SK;Jones DL
通讯作者：
Jones DL
影响因子：
12.8
作者：
Brunner, F. S.;Brown, M. R.;Bassano, I;Denise, H.;Khalifa, M. S.;Wade, M. J.;van Aerle, R.;Kevill, J. L.;Jones, D. L.;Farkas, K.;Jeffries, A. R.;Cairns, E.;Wierzbicki, C.;Paterson, S.
通讯作者：
Paterson, S.
影响因子：
3.7
作者：
Farkas, Kata;Pellett, Cameron;Alex-Sanders, Natasha;Bridgman, Matthew T. P.;Corbishley, Alexander;Grimsley, Jasmine M. S.;Kasprzyk-Hordern, Barbara;Kevill, Jessica L.;Pantea, Igor;Richardson-O'Neill, India S.;Lambert-Slosarska, Kathryn;Woodhall, Nick;Jones, Davey L.
通讯作者：
Jones, Davey L.
DOI：
10.1021/acsestwater.1c00050
发表时间：
2021-05-10
期刊：
ACS ES&T WATER
影响因子：
--
作者：
Fuschi, Claire;Pu, Haihui;Chen, Junhong
通讯作者：
Chen, Junhong