Surveillance of SARS-CoV-2 RNA in wastewater: Methods optimisation and quality control are crucial for generating reliable public health information.
Surveillance of SARS-CoV-2 RNA in wastewater: Methods optimisation and quality control are crucial for generating reliable public health information.
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废水中SARS-CoV-2 RNA的监测：方法优化和质量控制对于生成可靠的公共卫生信息至关重要。
DOI：
10.1016/j.coesh.2020.09.003
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发表时间：
2020-09-30
影响因子：
--
通讯作者：
Mueller JF
中科院分区：
文献类型：
--
作者：
Ahmed W;Bivins A;Bertsch PM;Bibby K;Choi PM;Farkas K;Gyawali P;Hamilton KA;Haramoto E;Kitajima M;Simpson SL;Tandukar S;Thomas K;Mueller JF
通过基于废水的流行病学 (WBE) 过程监测废水中的 SARS-CoV-2 RNA 提供了一种额外的监测工具，有助于基于社区的筛查和预防工作，因为这些测量在某些情况下是在疾病病例发生之前进行的。全球范围内已报道了使用各种方法检测到 SARS-CoV-2 RNA 的大量报告，证明了常规监测的技术可行性。然而，为了可靠地解释这些努力产生的数据，为公共卫生干预措施提供信息，需要额外的质量控制信息以及抽样设计、样本处理、数据解释和报告方面的标准化。本综述总结了已发表的废水中 SARS-CoV-2 RNA 检测研究以及有关浓度、提取和检测方法的可用信息。该审查强调了潜在标准化的领域，包括与粪便装载峰值时间相关的采样时间和频率相关的考虑因素；纳入有关所收集样本量的适当信息；样本采集点；运输和储存条件；样品浓缩和处理； RNA提取工艺及性能；有效体积； PCR抑制；整个样品采集和处理过程的过程控制； PCR标准曲线性能；以及回收效率测试。建议研究人员遵循定量实时 PCR 发布的最低信息 (MIQE) 指南。遵守这些建议将能够对废水监测结果进行强有力的解释，并改进关于监测结果与疾病病例之间关系的推论。
Monitoring for SARS-CoV-2 RNA in wastewater through the process of wastewater-based epidemiology (WBE) provides an additional surveillance tool, contributing to community-based screening and prevention efforts as these measurements have preceded disease cases in some instances. Numerous detections of SARS-CoV-2 RNA have been reported globally using various methods, demonstrating the technical feasibility of routine monitoring. However, in order to reliably interpret data produced from these efforts for informing public health interventions, additional quality control information and standardization in sampling design, sample processing, and data interpretation and reporting is needed. This review summarizes published studies of SARS-CoV-2 RNA detection in wastewater as well as available information regarding concentration, extraction, and detection methods. The review highlights areas for potential standardization including considerations related to sampling timing and frequency relative to peak fecal loading times; inclusion of appropriate information on sample volume collected; sample collection points; transport and storage conditions; sample concentration and processing; RNA extraction process and performance; effective volumes; PCR inhibition; process controls throughout sample collection and processing; PCR standard curve performance; and recovery efficiency testing. Researchers are recommended to follow the Minimum Information for Publication of Quantitative Real-Time PCR (MIQE) guidelines. Adhering to these recommendations will enable robust interpretation of wastewater monitoring results and improved inferences regarding the relationship between monitoring results and disease cases.
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DOI：
10.1016/j.ijfoodmicro.2015.02.029
发表时间：
2015-06-02
影响因子：
5.4
作者：
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