Feedback inhibition of immunoglobulin gene rearrangement by membrane mu, but not by secreted mu heavy chains.
Feedback inhibition of immunoglobulin gene rearrangement by membrane mu, but not by secreted mu heavy chains.
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膜MU对免疫球蛋白基因重排的反馈抑制，而不是分泌的MU重链。
DOI：
10.1084/jem.168.4.1363
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发表时间：
1988-10-01
影响因子：
15.3
通讯作者：
STORB, U
中科院分区：
文献类型：
--
作者：
MANZ, J;DENIS, K;WITTE, O;BRINSTER, R;STORB, U
先前的研究（6-10）表明，Ig基因表达的等位基因排除受mu和kappa基因功能重排的控制。本报告比较了膜mu（微米）和分泌mu（微秒）在促进这种反馈抑制中的作用。我们分析了单独携带重排kappa基因或与完整重排mu基因或截断的mu基因结合的转基因小鼠的脾B细胞杂交瘤。完整的mu基因能够产生膜和分泌形式的蛋白质，而截断的mu基因只能编码分泌形式的蛋白质。在b前细胞系中也测试了微秒的作用。内源性Ig基因重排程度分析表明(a) micron和kappa的产生可以终止Ig基因重排；(b)微秒与kappa没有这种反馈效应；(c)微秒可能干扰微米和卡帕效应；(d)这里显示的反馈可能代表micron + kappa完全关闭了特定的重组酶；这些数据并没有解决mu单独影响H基因重排的可及性的问题。
Previous work (6-10) has shown that allelic exclusion of Ig gene expression is controlled by functionally rearranged mu and kappa genes. This report deals with the comparison of membrane mu (micron) and secreted mu (microsecond) in promoting such feedback inhibition. Splenic B cell hybridomas were analyzed from transgenic mice harboring a rearranged kappa gene alone or in combination with either an intact rearranged mu gene or a truncated version of the mu gene. The intact mu gene is capable of producing both membrane and secreted forms of the protein, while the truncated version can only encode the secreted form. The role of the microsecond was also tested in pre-B cell lines. Analysis of the extent of endogenous Ig gene rearrangement revealed that (a) the production of micron together with kappa can terminate Ig gene rearrangement; (b) microsecond with kappa does not have this feedback effect; (c) microsecond may interfere with the effect of micron and kappa; and (d) the feedback shown here probably represents a complete shutoff of the specific recombinase by micron + kappa; the data do not address the question of mu alone affecting the accessibility of H genes for rearrangement.
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影响因子：
64.5
作者：
EARLY, P;ROGERS, J;HOOD, L
通讯作者：
HOOD, L
影响因子：
14.9
作者：
KELLEY, DE;PERRY, RP
通讯作者：
PERRY, RP
DOI：
10.1073/pnas.77.12.7400
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
KEMP, DJ;HARRIS, AW;ADAMS, JM
通讯作者：
ADAMS, JM
影响因子：
10.5
作者：
LIEBER, MR;HESSE, JE;GELLERT, M
通讯作者：
GELLERT, M
影响因子：
56.9
作者：
NUSSENZWEIG, MC;SHAW, AC;LEDER, P
通讯作者：
LEDER, P