Genetic diversity and population structure of Schistosoma mansoni within human infrapopulations in Mwea, central Kenya assessed by microsatellite markers.
Genetic diversity and population structure of Schistosoma mansoni within human infrapopulations in Mwea, central Kenya assessed by microsatellite markers.
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DOI：
10.1016/j.actatropica.2009.04.012
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发表时间：
2009-09
期刊：
影响因子：
2.7
通讯作者：
Mkoji GM
中科院分区：
文献类型：
--
作者：
Agola LE;Steinauer ML;Mburu DN;Mungai BN;Mwangi IN;Magoma GN;Loker ES;Mkoji GM
最近开发的高通量技术，允许多位点微卫星分析的个人毛蚴的曼氏血吸虫被用来评估的遗传多样性和人口结构的水平，在12 infropouples的寄生虫，每个infropouples来自一个受感染的学童从Mwea地区，肯尼亚中部。每个基因座的平均等位基因数在8.22 - 10.22之间，Hardy-Weinberg平衡的期望杂合度为0.68 - 0.70，12个亚群的成对FST值在0.16 - 3.98%之间。虽然各亚种群内的遗传多样性差异很大。在该地区的mansoni一般高，低水平的遗传结构观察，暗示高水平的基因流之间的亚种群。在12个亚群中，有8个个体存在私有等位基因，每个亚群中最高的私有等位基因数为3个。我们的数据表明，S亚种群之间的基因流水平。因此，Mwea中的mansoni非常高，为稀有等位基因的传播提供了机会，包括那些可能赋予诸如耐药性和毒力等性状的等位基因。
A recently developed high-throughput technique that allows multi-locus microsatellite analysis of individual miracidia of Schistosoma mansoni was used to assess the levels of genetic diversity and population structure in 12 infrapopulations of the parasite, each infrapopulation derived from an infected school child from the Mwea area, central Kenya. The mean number of alleles per locus was in the range 8.22 – 10.22, expected heterozygosity in Hardy-Weinberg equilibrium was 0.68 – 0.70, and pair wise FST values ranged from 0.16 −3.98% for the 12 infrapopulations. Although the genetic diversity within each infrapopulation of S. mansoni in this area was generally high, low levels of genetic structure were observed, suggestive of high levels of gene flow among infrapopulations. Private alleles were found in 8 of the 12 infrapopulation, the highest number of private alleles recorded per infrapopulation was 3. Our data suggest that the level of gene flow among infrapopulations of S. mansoni in Mwea is extremely high thus, providing opportunity for spread of rare alleles, including those that may confer character traits such as drug resistance and virulence.
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