Isolation of SARS-CoV-2 in Viral Cell Culture in Immunocompromised Patients With Persistently Positive RT-PCR Results.
Isolation of SARS-CoV-2 in Viral Cell Culture in Immunocompromised Patients With Persistently Positive RT-PCR Results.
复制标题
DOI：
10.3389/fcimb.2022.804175
复制
发表时间：
2022
影响因子：
5.7
通讯作者：
Kwon JH
中科院分区：
文献类型：
--
作者：
Sung A;Bailey AL;Stewart HB;McDonald D;Wallace MA;Peacock K;Miller C;Reske KA;O'Neil CA;Fraser VJ;Diamond MS;Burnham CD;Babcock HM;Kwon JH
在初次诊断 2019 年冠状病毒病 (COVID-19) 很久之后，免疫功能低下的成年人可能会长期出现急性呼吸综合征冠状病毒 2 (SARS-CoV-2) RT-PCR 阳性结果。本研究旨在确定是否可以从 SARS-CoV-2 RT-PCR 检测持续呈阳性的免疫功能低下成年人的病毒细胞培养物中回收 SARS-CoV-2 病毒。我们从 20 名免疫功能低下的成年人那里获得了 20 份残留的 SARS-CoV-2 PCR 阳性鼻咽拭子，这些拭子在初次检测呈阳性后 ≥14 天呈 RT-PCR 检测呈阳性。患者的第二份检测样本进行了SARS-CoV-2抗原检测，并用Vero-hACE2-TMPRSS2细胞进行培养。经过 qRT-PCR 和噬菌斑测定后，从培养的细胞中回收病毒 RNA 和可培养的病毒。 20 名患者中，10 名（50%）接受了实体器官移植，5 名（25%）患有血液恶性肿瘤。对于大多数患者，RT-PCR Ct 值随着时间的推移而增加。有2例患者病毒细胞培养呈阳性；一名接受 Venetoclax 和 obinutuzumab 治疗的慢性淋巴细胞白血病患者的病毒滴度较低，为 27 PFU/mL。第二名患者患有边缘区淋巴瘤，接受苯达莫司汀和利妥昔单抗治疗，病毒滴度高达 2 x 106 PFU/mL。最初 SARS-CoV-2 RT-PCR 呈阳性后 7 天以上收集的大多数样本的病毒细胞培养物呈阴性。 2 名病毒细胞培养呈阳性的患者患有血液恶性肿瘤，并接受了化疗和 B 细胞耗竭疗法。一名患者的可培养病毒滴度高。需要进一步的数据来确定持续病毒脱落的危险因素以及防止免疫功能低下宿主传播 SARS-CoV-2 的方法。
Immunocompromised adults can have prolonged acute respiratory syndrome coronavirus 2 (SARS-CoV-2) positive RT-PCR results, long after the initial diagnosis of coronavirus disease 2019 (COVID-19). This study aimed to determine if SARS-CoV-2 virus can be recovered in viral cell culture from immunocompromised adults with persistently positive SARS-CoV-2 RT-PCR tests. We obtained 20 remnant SARS-CoV-2 PCR positive nasopharyngeal swabs from 20 immunocompromised adults with a positive RT-PCR test ≥14 days after the initial positive test. The patients’ 2nd test samples underwent SARS-CoV-2 antigen testing, and culture with Vero-hACE2-TMPRSS2 cells. Viral RNA and cultivable virus were recovered from the cultured cells after qRT-PCR and plaque assays. Of 20 patients, 10 (50%) had a solid organ transplant and 5 (25%) had a hematologic malignancy. For most patients, RT-PCR Ct values increased over time. There were 2 patients with positive viral cell cultures; one patient had chronic lymphocytic leukemia treated with venetoclax and obinutuzumab who had a low viral titer of 27 PFU/mL. The second patient had marginal zone lymphoma treated with bendamustine and rituximab who had a high viral titer of 2 x 106 PFU/mL. Most samples collected ≥7 days after an initial positive SARS-CoV-2 RT-PCR had negative viral cell cultures. The 2 patients with positive viral cell cultures had hematologic malignancies treated with chemotherapy and B cell depleting therapy. One patient had a high concentration titer of cultivable virus. Further data are needed to determine risk factors for persistent viral shedding and methods to prevent SARS-CoV-2 transmission from immunocompromised hosts.
登录
查看更多内容
DOI：
10.1016/j.diagmicrobio.2021.115387
发表时间：
2021-10
影响因子：
2.9
作者：
Potter, Robert F.;Abro, Brooj;Eby, Charles S.;Burnham, Carey-Ann D.;Anderson, Neil W.;Parikh, Bijal A.
通讯作者：
Parikh, Bijal A.
影响因子：
11.1
作者：
Lu, Jing;Peng, Jinju;Ke, Changwen
通讯作者：
Ke, Changwen
影响因子：
11.8
作者：
Bullard, Jared;Dust, Kerry;Poliquin, Guillaume
通讯作者：
Poliquin, Guillaume
DOI：
10.1007/s10096-020-03913-9
发表时间：
2020-04-27
影响因子：
4.5
作者：
La Scola, Bernard;Le Bideau, Marion;Raoult, Didier
通讯作者：
Raoult, Didier
影响因子：
82.9
作者：
He, Xi;Lau, Eric H. Y.;Leung, Gabriel M.
通讯作者：
Leung, Gabriel M.