A de novo transcriptome of the Asian tiger mosquito, Aedes albopictus, to identify candidate transcripts for diapause preparation.
A de novo transcriptome of the Asian tiger mosquito, Aedes albopictus, to identify candidate transcripts for diapause preparation.
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DOI：
10.1186/1471-2164-12-619
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发表时间：
2011-12-20
期刊：
影响因子：
4.4
通讯作者：
Armbruster PA
中科院分区：
文献类型：
--
作者：
Poelchau MF;Reynolds JA;Denlinger DL;Elsik CG;Armbruster PA
许多温带昆虫通过经历光周期滞育（一种由短日照长度引发的预先编程的发育停滞）来在冬季的严酷条件下生存。尽管光周期滞育具有明确的生态意义，但这种关键适应的分子基础在很大程度上仍未得到解决。亚洲虎蚊白纹伊蚊（Skuse）代表了一种杰出的新兴模型，可以在明确的生态和进化背景下研究光周期滞育的分子基础。艾。白纹伊蚊是一种具有重要医学意义的媒介，目前被认为是世界上最具侵入性的蚊子。与滞育相关的特征似乎是导致这种蚊子快速传播的重要因素。为了生成该物种的新序列信息，以及发现滞育准备过程中涉及的转录本，我们对伊蚊的转录组进行了测序。白纹伊蚊卵母细胞注定会成为滞育或非滞育的噬菌体幼虫。 454 GS-FLX 转录组测序产生了超过 110 万个经过质量过滤的读数，我们将其组装成 69,474 个重叠群 (N50 = 1,009 bp)。我们的重叠群过滤方法利用了与埃及伊蚊以及其他参考生物体的完全测序基因组的高度相似性，产生了 11,561 个高质量、保守的 EST。基于标准化读数计数的差异表达估计显示，在滞育诱导条件下，57 个基因表达较高，257 个基因表达较低。通过 qPCR 对其中 47 个基因的表达进行分析表明，454 个序列数据与 qPCR 之间的表达水平具有高度相关性，但统计学上显着的差异表达的一致性较低。基于 qPCR 鉴定为差异表达的 7 个基因具有与昆虫滞育综合征一致的推定功能；三个基因的功能未知，代表了滞育转录基础的新候选基因。我们的转录组数据库为伊蚊的比较基因组学和功能遗传学提供了丰富的资源。白纹伊蚊，一种具有重要医学意义的入侵性蚊子。此外，与滞育相关的差异表达转录本的鉴定丰富了昆虫滞育分子基础的有限知识库，特别是准备阶段。最后，我们的分析说明了一种有用的方法，该方法利用密切相关的参考基因组在非模型生物体中生成高置信度的 EST。
Many temperate insects survive the harsh conditions of winter by undergoing photoperiodic diapause, a pre-programmed developmental arrest initiated by short day lengths. Despite the well-established ecological significance of photoperiodic diapause, the molecular basis of this crucial adaptation remains largely unresolved. The Asian tiger mosquito, Aedes albopictus (Skuse), represents an outstanding emerging model to investigate the molecular basis of photoperiodic diapause in a well-defined ecological and evolutionary context. Ae. albopictus is a medically significant vector and is currently considered the most invasive mosquito in the world. Traits related to diapause appear to be important factors contributing to the rapid spread of this mosquito. To generate novel sequence information for this species, as well as to discover transcripts involved in diapause preparation, we sequenced the transcriptome of Ae. albopictus oocytes destined to become diapausing or non-diapausing pharate larvae. 454 GS-FLX transcriptome sequencing yielded >1.1 million quality-filtered reads, which we assembled into 69,474 contigs (N50 = 1,009 bp). Our contig filtering approach, where we took advantage of strong sequence similarity to the fully sequenced genome of Aedes aegypti, as well as other reference organisms, resulted in 11,561 high-quality, conservative ESTs. Differential expression estimates based on normalized read counts revealed 57 genes with higher expression, and 257 with lower expression under diapause-inducing conditions. Analysis of expression by qPCR for 47 of these genes indicated a high correlation of expression levels between 454 sequence data and qPCR, but congruence of statistically significant differential expression was low. Seven genes identified as differentially expressed based on qPCR have putative functions that are consistent with the insect diapause syndrome; three genes have unknown function and represent novel candidates for the transcriptional basis of diapause. Our transcriptome database provides a rich resource for the comparative genomics and functional genetics of Ae. albopictus, an invasive and medically important mosquito. Additionally, the identification of differentially expressed transcripts related to diapause enriches the limited knowledge base for the molecular basis of insect diapause, in particular for the preparatory stage. Finally, our analysis illustrates a useful approach that draws from a closely related reference genome to generate high-confidence ESTs in a non-model organism.
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影响因子：
7.8
作者：
Alic N;Hoddinott MP;Vinti G;Partridge L
通讯作者：
Partridge L
鉴定与棉铃虫大脑中滞育开始相关的基因表达变化
影响因子：
4.4
作者：
Bao B;Xu WH
通讯作者：
Xu WH
影响因子：
2.1
作者：
Benedict, Mark Q.;Levine, Rebecca S.;Lounibos, L. Philip
通讯作者：
Lounibos, L. Philip
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
1.3
作者：
Denlinger, David L.
通讯作者：
Denlinger, David L.