Identification of gene expression changes associated with the initiation of diapause in the brain of the cotton bollworm, Helicoverpa armigera.
Identification of gene expression changes associated with the initiation of diapause in the brain of the cotton bollworm, Helicoverpa armigera.
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鉴定与棉铃虫大脑中滞育开始相关的基因表达变化
DOI：
10.1186/1471-2164-12-224
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发表时间：
2011-05-11
期刊：
影响因子：
4.4
通讯作者：
Xu WH
中科院分区：
文献类型：
--
作者：
Bao B;Xu WH
滞育是一种发育停滞并伴随代谢率显著降低的状态，有助于昆虫克服不利的季节。棉铃虫（Helicoverpaarmigera，Har）蛹经历滞育，但滞育起始的分子机制尚不清楚。利用抑制性消减杂交（SSH），我们研究了滞育和非滞育蛹脑中的差异表达基因在滞育开始。我们构建了两个SSH文库（正向，F和反向，R），以分离在滞育起始时上调或下调的基因。我们在F文库中获得了194个独特序列，在R文库中获得了115个独特序列。此外，基因表达在mRNA和蛋白质水平上滞育和非滞育的蛹注定的大脑中，证实了通过RT-PCR，北方印迹或Western印迹分析。最后，我们对这些基因进行了分类，并预测了它们在滞育起始中可能的作用。蛹滞育起始期差异表达的基因可能参与代谢、能量、抗逆、信号转导、细胞周期、转录和翻译等方面的调控。
Diapause, a state of arrested development accompanied by a marked decrease of metabolic rate, helps insects to overcome unfavorable seasons. Helicoverpa armigera (Har) undergoes pupal diapause, but the molecular mechanism of diapause initiation is unclear. Using suppression subtractive hybridization (SSH), we investigated differentially expressed genes in diapause- and nondiapause-destined pupal brains at diapause initiation. We constructed two SSH libraries (forward, F and reverse, R) to isolate genes that are up-regulated or down-regulated at diapause initiation. We obtained 194 unique sequences in the F library and 115 unique sequences in the R library. Further, genes expression at the mRNA and protein level in diapause- and nondiapause-destined pupal brains were confirmed by RT-PCR, Northern blot or Western blot analysis. Finally, we classified the genes and predicted their possible roles at diapause initiation. Differentially expressed genes at pupal diapause initiation are possibly involved in the regulation of metabolism, energy, stress resistance, signaling pathways, cell cycle, transcription and translation.
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DOI：
10.1073/pnas.0408945102
发表时间：
2005-08-16
影响因子：
11.1
作者：
Bellosta, P;Hulf, T;Gallant, P
通讯作者：
Gallant, P
DOI：
10.1073/pnas.95.10.5616
发表时间：
1998-05-12
影响因子：
11.1
作者：
Flannagan, RD;Tammariello, SP;Denlinger, DL
通讯作者：
Denlinger, DL
棉铃虫 Helicoverpa armigera 滞育起始时的蛋白质组和磷酸蛋白质组分析
影响因子：
4.4
作者：
Lu, Yu-Xuan;Xu, Wei-Hua
通讯作者：
Xu, Wei-Hua
影响因子：
1.3
作者：
Kurban, A;Yoshida, H;Tsumuki, H
通讯作者：
Tsumuki, H
影响因子：
3.7
作者：
Emerson KJ;Bradshaw WE;Holzapfel CM
通讯作者：
Holzapfel CM