Metabolite Profiling Reveals Distinct Modulation of Complex Metabolic Networks in Non-Pigmented, Black, and Red Rice (Oryza sativa L.) Cultivars.
Metabolite Profiling Reveals Distinct Modulation of Complex Metabolic Networks in Non-Pigmented, Black, and Red Rice (Oryza sativa L.) Cultivars.
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DOI：
10.3390/metabo11060367
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发表时间：
2021-06-09
期刊：
影响因子：
4.1
通讯作者：
Kim JK
中科院分区：
文献类型：
--
作者：
Kim TJ;Kim SY;Park YJ;Lim SH;Ha SH;Park SU;Lee B;Kim JK
为了解色素水稻（Oryza sativa L.）颜色形成的代谢差异，对其初级和次级代谢产物进行了全面分析。总的来说，从非色素水稻、黑稻和红稻品种中鉴定出110种代谢物。黑米和红米含有大量与植物颜色相关的类黄酮。黑米还含有大量的萜类物质（类胡萝卜素、生育酚、植物甾醇和单萜类物质）。非色素水稻的次生代谢物含量相对较低。进行多变量和途径分析，以数据挖掘代谢物谱。相关系数的分层聚类分析揭示了基于氮和碳源的代谢物聚类。这些簇表明氮和碳之间呈负相关。途径分析表明，黑米富含碳基次生代谢物，与其他水稻品种相比，其初级代谢物水平相对较低。这些数据强调了水稻初级代谢物和次级代谢物氮碳代谢之间复杂的相互作用。首次分析了非色素水稻品种和色素水稻品种间萜类（单萜、三萜和四萜）含量的关系和代谢差异。这些发现将对色素水稻代谢组的理解和水稻新品种的选育具有重要意义。
Comprehensive profiling of primary and secondary metabolites was performed to understand metabolic differences associated with color formation in pigmented rice (Oryza sativa L.). Overall, 110 metabolites from non-pigmented, black, and red rice cultivars were identified. Black and red rice contained high levels of flavonoids associated with plant color. Black rice also contained high levels of terpenoids (carotenoids, tocopherols, phytosterols, and monoterpenes). The non-pigmented rice contained relatively low levels of secondary metabolites. Multivariate and pathway analyses were performed to data-mine the metabolite profiles. Hierarchical clustering analysis of correlation coefficients revealed metabolite clusters based on nitrogen and carbon sources. These clusters suggested a negative correlation between nitrogen and carbon. Pathway analysis revealed that black rice was rich in carbon-based secondary metabolites, with relatively low levels of primary metabolites compared with other rice cultivars. These data highlight the complex interactions between nitrogen and carbon metabolism of primary and secondary metabolites in rice. For the first time, the relationships and metabolic differences in terpenoid content (monoterpenes, triterpenes, and tetraterpenes) of non-pigmented and pigmented rice cultivars were analyzed. These findings should greatly contribute to the understanding of pigmented rice metabolome and inform breeding programs for new rice cultivars.
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影响因子：
3.2
作者：
Choi, Sehun;Seo, Han-Seok;Lee, Jihyun
通讯作者：
Lee, Jihyun
DOI：
10.1007/s13765-015-0119-6
发表时间：
2015-12-01
影响因子：
--
作者：
Kim, Tae Jin;Lee, Kyoung Bok;Kim, Jae Kwang
通讯作者：
Kim, Jae Kwang
影响因子：
0.8
作者：
Lee, Ji Hae;Ham, Hyeonmi;Woo, Koan Sik
通讯作者：
Woo, Koan Sik
影响因子：
5.3
作者：
Payyavula RS;Navarre DA;Kuhl JC;Pantoja A;Pillai SS
通讯作者：
Pillai SS
影响因子：
8.8
作者：
Kim, Tae Jin;Hyeon, Hyejin;Kim, Jae Kwang
通讯作者：
Kim, Jae Kwang