Differential effects of environment on potato phenylpropanoid and carotenoid expression.
Differential effects of environment on potato phenylpropanoid and carotenoid expression.
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DOI：
10.1186/1471-2229-12-39
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发表时间：
2012-03-20
影响因子：
5.3
通讯作者：
Pillai SS
中科院分区：
文献类型：
--
作者：
Payyavula RS;Navarre DA;Kuhl JC;Pantoja A;Pillai SS
植物次生代谢物，包括苯丙素类和类胡萝卜素，是胁迫诱导的产物，在马铃薯生理中具有重要作用，影响马铃薯的营养价值。环境对块茎植物营养的影响的类型和大小尚不清楚，特别是在将压力降至最低的现代农业管理下。了解影响块茎次生代谢的因素有助于生产更有营养的作物。在北美(阿拉斯加、德克萨斯和佛罗里达)环境不同的地点种植的高苯丙醇紫色土豆中，测量了40多种块茎类苯丙素和类胡萝卜素的代谢物库以及20种结构基因的表达。包括绿原酸(CGA)在内的苯丙烷类化合物在北纬地区的样品中含量较高，苯丙氨酸解氨酶(PAL)等苯丙烷类基因的表达水平也较高，其相对丰度相差10倍以上。除羟基肉桂酰辅酶A：五酸羟基肉桂酰基转移酶(HQT；r=-0.24)外，苯丙烷类基因的表达与代谢物库呈协同调节，并与代谢物库密切相关。在电子启动子分析中发现了HQT启动子中的两个顺式作用元件，这些元件在其他苯丙烷类基因中没有发现。花色苷在阿拉斯加样品中含量较高，并与类黄酮基因DFR(r=0.91)、UFGT(r=0.94)和F3H(r=0.77)相关。花色苷含量最高的是Petunidin-3-Coum-rutinoside-5-Glu，在阿拉斯加为4.7 mg g-1，在德克萨斯州为2.3 mg g-1。块茎蔗糖与花色苷呈正相关(r=0.85)，提示蔗糖具有促进作用。观察到总类胡萝卜素的变化较小，但个别类胡萝卜素的差异显著，其变化范围超过十倍。紫黄质、叶黄素和玉米黄素分别是阿拉斯加、德克萨斯和佛罗里达块茎中的主要类胡萝卜素。与苯丙烷途径不同，类胡萝卜素转录物和代谢物之间的相关性很差。对块茎次生代谢的分析表明，不同代谢物之间存在有趣的关系，以响应集体环境影响，即使在最大限度地减少胁迫的条件下也是如此。代谢产物的变化表明了块茎次生代谢可能存在的相当大的表型可塑性，并提出了这样的问题：这些途径在多大程度上可以被环境线索刺激，从而在保护产量的同时优化块茎植物营养成分的含量。次生代谢物的差异可能足以影响营养质量。
Plant secondary metabolites, including phenylpropanoids and carotenoids, are stress inducible, have important roles in potato physiology and influence the nutritional value of potatoes. The type and magnitude of environmental effects on tuber phytonutrients is unclear, especially under modern agricultural management that minimizes stress. Understanding factors that influence tuber secondary metabolism could facilitate production of more nutritious crops. Metabolite pools of over forty tuber phenylpropanoids and carotenoids, along with the expression of twenty structural genes, were measured in high-phenylpropanoid purple potatoes grown in environmentally diverse locations in North America (Alaska, Texas and Florida). Phenylpropanoids, including chlorogenic acid (CGA), were higher in samples from the northern latitudes, as was the expression of phenylpropanoid genes including phenylalanine ammonia lyase (PAL), which had over a ten-fold difference in relative abundance. Phenylpropanoid gene expression appeared coordinately regulated and was well correlated with metabolite pools, except for hydroxycinnamoyl-CoA:quinatehydroxcinnamoyl transferase (HQT; r = -0.24). In silico promoter analysis identified two cis-acting elements in the HQT promoter not found in the other phenylpropanoid genes. Anthocyanins were more abundant in Alaskan samples and correlated with flavonoid genes including DFR (r = 0.91), UFGT (r = 0.94) and F3H (r = 0.77). The most abundant anthocyanin was petunidin-3-coum-rutinoside-5-glu, which ranged from 4.7 mg g-1 in Alaska to 2.3 mg g-1 in Texas. Positive correlations between tuber sucrose and anthocyanins (r = 0.85), suggested a stimulatory effect of sucrose. Smaller variation was observed in total carotenoids, but marked differences occurred in individual carotenoids, which had over a ten-fold range. Violaxanthin, lutein or zeaxanthin were the predominant carotenoids in tubers from Alaska, Texas and Florida respectively. Unlike in the phenylpropanoid pathway, poor correlations occurred between carotenoid transcripts and metabolites. Analysis of tuber secondary metabolism showed interesting relationships among different metabolites in response to collective environmental influences, even under conditions that minimize stress. The variation in metabolites shows the considerable phenotypical plasticity possible with tuber secondary metabolism and raises questions about to what extent these pathways can be stimulated by environmental cues in a manner that optimizes tuber phytonutrient content while protecting yields. The differences in secondary metabolites may be sufficient to affect nutritional quality.
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影响因子：
7.4
作者：
Diretto, Gianfranco;Al-Babili, Salim;Giuliano, Giovanni
通讯作者：
Giuliano, Giovanni
影响因子：
7.3
作者：
DEMMIGADAMS, B;ADAMS, WW
通讯作者：
ADAMS, WW
影响因子：
7.3
作者：
Grace, SC;Logan, BA;Adams, WW
通讯作者：
Adams, WW
影响因子：
4.1
作者：
Chun, OK;Kim, DO;Lee, CY
通讯作者：
Lee, CY
影响因子：
4.3
作者：
CHRISTIE, PJ;ALFENITO, MR;WALBOT, V
通讯作者：
WALBOT, V