Longitudinal comparison of the developing gut virome in infants and their mothers.
Longitudinal comparison of the developing gut virome in infants and their mothers.
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DOI：
10.1016/j.chom.2023.01.003
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发表时间：
2023-02-08
影响因子：
30.3
通讯作者：
Chiu, Charles Y.
中科院分区：
文献类型：
--
作者：
Walters, William A.;Granados, Andrea C.;Ley, Catherine;Federman, Scot;Stryke, Doug;Santos, Yale;Haggerty, Thomas;Sotomayor-Gonzalez, Alicia;Servellita, Venice;Ley, Ruth E.;Parsonnet, Julie;Chiu, Charles Y.
人类肠道病毒组及其早期生命发育尚不清楚。先前的研究已经捕获了单点评估，而婴儿病毒的进化在很大程度上仍未被探索。我们对53名2周至3岁的婴儿（807亿reads）及其母亲（98亿reads）的粪便样本进行了病毒宏基因组测序，以检查和比较病毒组。无症状婴儿病毒组包括噬菌体、非人类饮食/环境病毒和人类宿主病毒，主要是小核糖核酸病毒。相比之下，人类宿主病毒在母体病毒中基本不存在。以前没有描述过，在母体病毒体中检测到序列发散的脊椎动物病毒，但没有在婴儿病毒体中检测到。随着婴儿年龄的增长，噬菌体成分进化成与母体病毒体相似，但到3岁时，人类宿主成分仍然与母体病毒体不同。因此，生命早期病毒的发育主要是由饮食、感染和环境因素决定的，而不是由母体直接获得的。对53名从出生到3岁的婴儿及其母亲进行了粪便病毒组分析。人类宿主病毒，特别是小核糖核酸病毒，在婴儿病毒组中普遍存在。在母体中检测到序列发散型病毒，但在婴儿病毒组中没有检测到。婴儿病毒组与母亲病毒组在3岁之前仍然不同，这表明婴儿病毒组不是由母亲获得的，而是由饮食、感染和环境因素决定的。
The human gut virome and its early life development are poorly understood. Prior studies have captured single-point assessments with the evolution of the infant virome remaining largely unexplored. We performed viral metagenomic sequencing on stool samples collected longitudinally from a cohort of 53 infants from age 2 weeks to 3 years (80.7 billion reads), and from their mothers (9.8 billion reads) to examine and compare viromes. The asymptomatic infant virome consisted of bacteriophages, nonhuman dietary/environmental viruses, and human-host viruses, predominantly picornaviruses. In contrast, human-host viruses were largely absent from the maternal virome. Previously undescribed, sequence-divergent vertebrate viruses were detected in the maternal but not infant virome. As infants aged, the phage component evolved to resemble the maternal virome, but by age 3, the human-host component remained dissimilar from the maternal virome. Thus, early life virome development is determined predominantly by dietary, infectious, and environmental factors rather than direct maternal acquisition. Stool virome analysis was performed on 53 infants from birth to age 3 and their mothers Human-host viruses, particularly picornaviruses, are prevalent in the infant virome Sequence-divergent viruses were detected in the maternal but not infant virome Virome development in early life is not determined by direct maternal acquisition Walters et al. examine the evolution of the infant virome in early life in comparison to the maternal virome. The infant virome remained dissimilar from the maternal virome to age 3, showing that the infant virome is not maternally acquired but is rather determined by dietary, infectious, and environmental factors.
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影响因子：
48
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通讯作者：
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影响因子：
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