The CckA-ChpT-CtrA phosphorelay system is regulated by quorum sensing and controls flagellar motility in the marine sponge symbiont Ruegeria sp. KLH11.
The CckA-ChpT-CtrA phosphorelay system is regulated by quorum sensing and controls flagellar motility in the marine sponge symbiont Ruegeria sp. KLH11.
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DOI：
10.1371/journal.pone.0066346
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Hill RT
中科院分区：
文献类型：
--
作者：
Zan J;Heindl JE;Liu Y;Fuqua C;Hill RT
细菌通过信号转导途径对其环境做出反应，通常是通过磷酸转移来控制特定基因表达的双组分型系统。磷酸化继电器来自双组分系统，但由额外的组分组成。新月柄杆菌中必需的cckA-chpT-ctrA磷酸化中继已被充分研究，并且在协调细胞周期、极性发育和鞭毛生物发生中是重要的。虽然cckA，chpT和ctrA同源物在Alphaproteobacteria中广泛存在，但相对较少了解它们在Rhododocellales的大型和生态重要的Rosebellae分支中的功能。本文研究了海绵共生体Ruegeria sp. KLH 11的cckA-chpT-ctrA系统。我们的研究结果表明，cckA，chpT和ctrA基因正控制鞭毛的生物合成。与C. crescentus中的cckA、chpT和ctrA基因是非必需的，不影响细菌生长。基因融合和转录本分析为ctrA自身调节和运动相关基因的控制提供了证据。在KLH 11中，鞭毛运动性由SsaRI系统和酰基高丝氨酸内酯（阿勒）群体感应控制。SsaR和长链AHLs是cckA，chpT和ctrA基因表达所必需的，在KLH 11中提供鞭毛运动和群体密度之间的调节联系。
Bacteria respond to their environment via signal transduction pathways, often two-component type systems that function through phosphotransfer to control expression of specific genes. Phosphorelays are derived from two-component systems but are comprised of additional components. The essential cckA-chpT-ctrA phosphorelay in Caulobacter crescentus has been well studied and is important in orchestrating the cell cycle, polar development and flagellar biogenesis. Although cckA, chpT and ctrA homologues are widespread among the Alphaproteobacteria, relatively few is known about their function in the large and ecologically significant Roseobacter clade of the Rhodobacterales. In this study the cckA-chpT-ctrA system of the marine sponge symbiont Ruegeria sp. KLH11 was investigated. Our results reveal that the cckA, chpT and ctrA genes positively control flagellar biosynthesis. In contrast to C. crescentus, the cckA, chpT and ctrA genes in Ruegeria sp. KLH11 are non-essential and do not affect bacterial growth. Gene fusion and transcript analyses provide evidence for ctrA autoregulation and the control of motility-related genes. In KLH11, flagellar motility is controlled by the SsaRI system and acylhomoserine lactone (AHL) quorum sensing. SsaR and long chain AHLs are required for cckA, chpT and ctrA gene expression, providing a regulatory link between flagellar locomotion and population density in KLH11.
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DOI：
10.1073/pnas.97.2.859
发表时间：
2000-01-18
影响因子：
11.1
作者：
Lang, AS;Beatty, JT
通讯作者：
Beatty, JT
影响因子：
3.6
作者：
Cheng Z;Miura K;Popov VL;Kumagai Y;Rikihisa Y
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Rikihisa Y
影响因子：
--
作者：
Brilli, Matteo;Fondi, Marco;Biondi, Emanuele G.
通讯作者：
Biondi, Emanuele G.
影响因子：
4.4
作者：
Khan, Sharik R.;Gaines, Jennifer;Farrand, Stephen K.
通讯作者：
Farrand, Stephen K.
影响因子：
56.9
作者：
Holtzendorff, J;Hung, D;Shapiro, L
通讯作者：
Shapiro, L