Insights into the CtrA regulon in development of stress resistance in obligatory intracellular pathogen Ehrlichia chaffeensis.
Insights into the CtrA regulon in development of stress resistance in obligatory intracellular pathogen Ehrlichia chaffeensis.
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DOI：
10.1111/j.1365-2958.2011.07885.x
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发表时间：
2011-12
影响因子：
3.6
通讯作者：
Rikihisa Y
中科院分区：
文献类型：
--
作者：
Cheng Z;Miura K;Popov VL;Kumagai Y;Rikihisa Y
查菲埃里希体是一种引起人类单核细胞埃里希体病的专性细胞内细菌。埃里希体具有由致密核心细胞（DCs）和网状细胞（RCs）组成的双相发育周期。分离的DC比RC更具应激抗性和感染性。在这里，我们报告说，反应调节，CtrA在人单核细胞在生长后期，当DC发展上调。E. chaffeensis CtrA结合到后期转录基因的启动子：ctrA，ompA（肽聚糖相关脂蛋白），bolA（应激诱导的形态发生蛋白），和surE（稳定期生存蛋白），其中包含CtrA结合基序，和反式激活ompA，surE，和bolA启动子-lacZ融合在大肠杆菌中。OmpA主要在DC中表达。E.抗OmpA IgG可抑制Chaffeensis与单核细胞的结合和随后的单核细胞感染。E. chaffeensis BolA结合编码外表面蛋白TRP 120和ECH_1038的基因的启动子，其在DC中表达，并反式激活trp 120和ECH_1038启动子-lacZ融合物。E. chaffeensis bolA补充了一个胁迫敏感的E. colibolA突变体。E. coli表达E. chaffeensis surE表现出增加的抗渗透胁迫。我们的结果表明，E. chaffeensis CtrA在协调从当前宿主细胞到下一个宿主细胞的应激抗性的发展中起作用。
Ehrlichia chaffeensis is an obligate intracellular bacterium that causes human monocytic ehrlichiosis. Ehrlichiae have a biphasic developmental cycle consisting of dense-cored cells (DCs) and reticulate cells (RCs). Isolated DCs are more stress resistant and infectious than RCs. Here, we report that a response regulator, CtrA was upregulated in human monocytes at the late growth stage when DCs develop. E. chaffeensis CtrA bound to the promoters of late-stage transcribed genes: ctrA, ompA (peptidoglycan-associated lipoprotein), bolA (stress-induced morphogen), and surE (stationary phase survival protein), which contain CtrA-binding motifs, and transactivated ompA, surE, and bolA promoter-lacZ fusions in Escherichia coli. OmpA was predominantly expressed in DCs. E. chaffeensis binding to and subsequent infection of monocytes were inhibited by anti-OmpA IgG. E. chaffeensis BolA bound to the promoters of genes encoding outer surface proteins TRP120 and ECH_1038, which were expressed in DCs, and transactivated trp120 and ECH_1038 promoter-lacZ fusions. E. chaffeensis bolA complemented a stress-sensitive E. coli bolA mutant. E. coli expressing E. chaffeensis surE exhibited increased resistance to osmotic stress. Our results suggest that E. chaffeensis CtrA plays a role in coordinating development of the stress resistance for passage from the present to the next host cells through its regulon.
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影响因子：
4.5
作者：
Dunning Hotopp JC;Lin M;Madupu R;Crabtree J;Angiuoli SV;Eisen JA;Seshadri R;Ren Q;Wu M;Utterback TR;Smith S;Lewis M;Khouri H;Zhang C;Niu H;Lin Q;Ohashi N;Zhi N;Nelson W;Brinkac LM;Dodson RJ;Rosovitz MJ;Sundaram J;Daugherty SC;Davidsen T;Durkin AS;Gwinn M;Haft DH;Selengut JD;Sullivan SA;Zafar N;Zhou L;Benahmed F;Forberger H;Halpin R;Mulligan S;Robinson J;White O;Rikihisa Y;Tettelin H
通讯作者：
Tettelin H
影响因子：
3.4
作者：
Cheng, Zhihui;Kumagai, Yumi;Rikihisa, Yasuko
通讯作者：
Rikihisa, Yasuko
影响因子：
2.1
作者：
Freire, Patrick;Vieira, Helena L. A.;Arraiano, Cecilia M.
通讯作者：
Arraiano, Cecilia M.
影响因子：
64.5
作者：
Domian, IJ;Quon, KC;Shapiro, L
通讯作者：
Shapiro, L
DOI：
10.1073/pnas.88.9.3937
发表时间：
1991-05-01
影响因子：
11.1
作者：
HACKSTADT, T;BAEHR, W;YING, Y
通讯作者：
YING, Y