The Role of α-CTD in the Genome-Wide Transcriptional Regulation of the Bacillus subtilis Cells.
The Role of α-CTD in the Genome-Wide Transcriptional Regulation of the Bacillus subtilis Cells.
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DOI：
10.1371/journal.pone.0131588
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Oshima T
中科院分区：
文献类型：
--
作者：
Murayama S;Ishikawa S;Chumsakul O;Ogasawara N;Oshima T
RNA聚合酶（RNAP）α亚基的氨基酸序列在真细菌中是非常保守的。它的C-末端结构域（α-CTD）通过与转录因子和/或称为"UP元件"的DNA元件相互作用，对大肠杆菌和枯草芽孢杆菌中特异性启动子的转录调节是重要的。然而，关于B中α-CTD调控基因的信息有限。枯草芽孢杆菌和该亚基的重要性，在转录调控的B。枯草杆菌。在这里，我们建立了菌株和生长条件，其中RNAP的α-亚基被替换为C-末端截短的版本。转录组学和ChAP芯片分析显示，α-CTD缺陷减少了与次级碳源利用、过渡态反应和核糖体合成相关的基因的转录和RNAP结合。在大肠在大肠杆菌中，α-CTD还参与了与二次碳源利用和核糖体合成相关基因的表达。我们的结果表明，α-CTD的生物学重要性是保守的B。枯草芽孢杆菌和E.大肠杆菌，但它的具体作用在这两种细菌之间已经多样化。
The amino acid sequence of the RNA polymerase (RNAP) α-subunit is well conserved throughout the Eubacteria. Its C-terminal domain (α-CTD) is important for the transcriptional regulation of specific promoters in both Escherichia coli and Bacillus subtilis, through interactions with transcription factors and/or a DNA element called the “UP element”. However, there is only limited information regarding the α-CTD regulated genes in B. subtilis and the importance of this subunit in the transcriptional regulation of B. subtilis. Here, we established strains and the growth conditions in which the α-subunit of RNAP was replaced with a C-terminally truncated version. Transcriptomic and ChAP-chip analyses revealed that α-CTD deficiency reduced the transcription and RNAP binding of genes related to the utilization of secondary carbon sources, transition state responses, and ribosome synthesis. In E. coli, it is known that α-CTD also contributes to the expression of genes related to the utilization of secondary carbon sources and ribosome synthesis. Our results suggest that the biological importance of α-CTD is conserved in B. subtilis and E. coli, but that its specific roles have diversified between these two bacteria.
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