Regulatory protein HilD stimulates Salmonella Typhimurium invasiveness by promoting smooth swimming via the methyl-accepting chemotaxis protein McpC.
Regulatory protein HilD stimulates Salmonella Typhimurium invasiveness by promoting smooth swimming via the methyl-accepting chemotaxis protein McpC.
复制标题
调节蛋白HilD通过甲基接受趋化蛋白McpC促进平滑游泳来刺激鼠伤寒沙门氏菌的侵袭力。
DOI：
10.1038/s41467-020-20558-6
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发表时间：
2021-01-13
影响因子：
16.6
通讯作者：
Steele-Mortimer O
中科院分区：
文献类型：
--
作者：
Cooper KG;Chong A;Kari L;Jeffrey B;Starr T;Martens C;McClurg M;Posada VR;Laughlin RC;Whitfield-Cargile C;Garry Adams L;Bryan LK;Little SV;Krath M;Lawhon SD;Steele-Mortimer O
在肠道病原体沙门氏菌肠道血清型鼠伤寒沙门氏菌中，入侵和运动由主调节因子HilD协调，其诱导III型分泌系统1（T3 SS 1）和运动基因的表达。甲基接受趋化蛋白（MCP）检测特定的配体并控制鞭毛马达的方向，促进翻滚和方向变化（如果检测到驱避剂）或平滑游泳（在引诱剂存在下）。在这里，我们表明，HilD诱导顺利游泳通过上调一个未知的MCP（McpC），这是重要的上皮细胞的入侵。值得注意的是，在体外试验表明，McpC可以抑制翻滚，并增加在没有外源性配体的情况下顺利游泳。mcpC的表达受到通用调节因子H-NS的抑制，H-NS可以被HilD取代。我们的研究结果强调了平滑游泳对鼠伤寒沙门氏菌侵袭性的重要性，并表明McpC可以通过配体独立的机制，当被纳入趋化受体阵列。鼠伤寒沙门氏菌的蛋白质HilD通过上调鞭毛基因和分泌系统来协调运动和宿主细胞入侵。在这里，库珀等人表明，HilD还通过上调趋化受体阵列的亚基来调节游泳行为，这对上皮细胞的侵袭很重要。
In the enteric pathogen Salmonella enterica serovar Typhimurium, invasion and motility are coordinated by the master regulator HilD, which induces expression of the type III secretion system 1 (T3SS1) and motility genes. Methyl-accepting chemotaxis proteins (MCPs) detect specific ligands and control the direction of the flagellar motor, promoting tumbling and changes in direction (if a repellent is detected) or smooth swimming (in the presence of an attractant). Here, we show that HilD induces smooth swimming by upregulating an uncharacterized MCP (McpC), and this is important for invasion of epithelial cells. Remarkably, in vitro assays show that McpC can suppress tumbling and increase smooth swimming in the absence of exogenous ligands. Expression of mcpC is repressed by the universal regulator H-NS, which can be displaced by HilD. Our results highlight the importance of smooth swimming for Salmonella Typhimurium invasiveness and indicate that McpC can act via a ligand-independent mechanism when incorporated into the chemotactic receptor array. Protein HilD of Salmonella Typhimurium coordinates motility and host cell invasion by upregulating flagellar genes and a secretion system. Here, Cooper et al. show that HilD also modulates swimming behaviour by upregulating a subunit of the chemotactic receptor array, and this is important for invasion of epithelial cells.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
3.6
作者：
Butler, SM;Nelson, EJ;Camilli, A
通讯作者：
Camilli, A
影响因子：
3.2
作者：
Feng, XH;Baumgartner, JW;Hazelbauer, GL
通讯作者：
Hazelbauer, GL
影响因子：
3.2
作者：
Chubiz, Jessica E. Cott;Golubeva, Yekaterina A.;Slauch, James M.
通讯作者：
Slauch, James M.
影响因子：
3.7
作者：
Hung CC;Haines L;Altier C
通讯作者：
Altier C