Role of hydrogen peroxide in competition and cooperation between Streptococcus gordonii and Actinomyces naeslundii.
Role of hydrogen peroxide in competition and cooperation between Streptococcus gordonii and Actinomyces naeslundii.
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DOI：
10.1111/j.1574-6941.2008.00585.x
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发表时间：
2008-12
影响因子：
4.2
通讯作者：
Kolenbrander PE
中科院分区：
文献类型：
--
作者：
Jakubovics NS;Gill SR;Vickerman MM;Kolenbrander PE
在牙菌斑中，α-溶血性链球菌，包括戈登链球菌，被认为对口腔健康有益。这些微生物产生的过氧化氢（H2O2）的浓度足以杀死许多口腔细菌。链球菌不产生过氧化氢酶，但耐受H2O2。我们最近证明了与naeslundii放线菌的共聚集稳定了S. gordonii的精氨酸生物合成。蛋白质精氨酸残基对H2O2的氧化很敏感。在这里，研究了纳斯伦迪对哥氏球菌（S. gordonii）对自产H2O2的保护能力。与A. naeslundii共聚集可以使S. gordonii在没有精氨酸的情况下生长，并且在添加或不添加精氨酸的情况下促进S. gordonii的存活。在指数生长过程中，富含精氨酸的单培养的H2O2浓度为20-30 μM。naeslundii放线菌不产生H2O2，但能合成过氧化氢酶，去除共聚集体培养物中的H2O2，并能降低S. gordonii的蛋白质氧化。在固体培养基上，金针菇抑制金针菇的生长；外源性过氧化氢酶克服了这种抑制。共聚集培养24 h后，褐藻细胞数量比单培养低约90%，表明与褐藻共聚集可保护褐藻免受氧化损伤。然而，高的细胞密度对纳氏拟虫有抑制作用。因此，H2O2可能会促使这些生物在天然牙菌斑中走向生态平衡的群落。
In dental plaque α-haemolytic streptococci, including Streptococcus gordonii, are considered beneficial for oral health. These organisms produce hydrogen peroxide (H2O2) at concentrations sufficient to kill many oral bacteria. Streptococci do not produce catalase yet tolerate H2O2. We recently demonstrated that coaggregation with Actinomyces naeslundii stabilizes arginine biosynthesis in S. gordonii. Protein arginine residues are sensitive to oxidation by H2O2. Here, the ability of A. naeslundii to protect S. gordonii against self-produced H2O2 was investigated. Coaggregation with A. naeslundii enabled S. gordonii to grow in the absence of arginine, and promoted survival of S. gordonii following growth with or without added arginine. Arginine-replete S. gordonii monocultures contained 20–30 μM H2O2 throughout exponential growth. Actinomyces naeslundii did not produce H2O2 but synthesized catalase, removed H2O2 from coaggregate cultures and decreased protein oxidation in S. gordonii. On solid medium, S. gordonii inhibited growth of A. naeslundii; exogenous catalase overcame this inhibition. In coaggregate cultures, A. naeslundii cell numbers were >90% lower than in monocultures after 24 h. These results indicate that coaggregation with A. naeslundii protects S. gordonii from oxidative damage. However, high cell densities of S. gordonii inhibit A. naeslundii. Therefore, H2O2 may drive these organisms towards an ecologically balanced community in natural dental plaque.
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DOI：
10.1099/13500872-140-12-3407
发表时间：
1994-12-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
BRADSHAW, DJ;HOMER, KA;BEIGHTON, D
通讯作者：
BEIGHTON, D
影响因子：
2.8
作者：
Jakubovics, NS;Smith, AW;Jenkinson, HF
通讯作者：
Jenkinson, HF
影响因子：
4.8
作者：
Dukan, S;Nyström, T
通讯作者：
Nyström, T
影响因子：
3.6
作者：
Lass, A;Suessenbacher, A;Brunner, F
通讯作者：
Brunner, F
影响因子：
3
作者：
MIYASAKI, KT;WILSON, ME;GENCO, RJ
通讯作者：
GENCO, RJ