Detection of SARS-associated coronavirus in throat wash and saliva in early diagnosis.
Detection of SARS-associated coronavirus in throat wash and saliva in early diagnosis.
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DOI：
10.3201/eid1007.031113
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发表时间：
2004-07
影响因子：
11.8
通讯作者：
SARS Research Group of the National Taiwan University/National Taiwan University Hospital
中科院分区：
文献类型：
--
作者：
Wang WK;Chen SY;Liu IJ;Chen YC;Chen HL;Yang CF;Chen PJ;Yeh SH;Kao CL;Huang LM;Hsueh PR;Wang JT;Sheng WH;Fang CT;Hung CC;Hsieh SM;Su CP;Chiang WC;Yang JY;Lin JH;Hsieh SC;Hu HP;Chiang YP;Wang JT;Yang PC;Chang SC;SARS Research Group of the National Taiwan University/National Taiwan University Hospital
Early detection of SARS-CoV in throat wash and saliva suggests that these specimens are ideal for SARS diagnosis. The severe acute respiratory syndrome–associated coronavirus (SARS-CoV) is thought to be transmitted primarily through dispersal of droplets, but little is known about the load of SARS-CoV in oral droplets. We examined oral specimens, including throat wash and saliva, and found large amounts of SARS-CoV RNA in both throat wash (9.58 x 102 to 5.93 x 106 copies/mL) and saliva (7.08 x 103 to 6.38 x 108 copies/mL) from all specimens of 17 consecutive probable SARS case-patients, supporting the possibility of transmission through oral droplets. Immunofluorescence study showed replication of SARS-CoV in the cells derived from throat wash, demonstrating the possibility of developing a convenient antigen detection assay. This finding, with the high detection rate a median of 4 days after disease onset and before the development of lung lesions in four patients, suggests that throat wash and saliva should be included in sample collection guidelines for SARS diagnosis.
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DOI：
10.1016/s0140-6736(03)13077-2
发表时间：
2003-04-19
期刊：
Lancet (London, England)
影响因子：
--
作者：
Peiris JS;Lai ST;Poon LL;Guan Y;Yam LY;Lim W;Nicholls J;Yee WK;Yan WW;Cheung MT;Cheng VC;Chan KH;Tsang DN;Yung RW;Ng TK;Yuen KY;SARS study group
通讯作者：
SARS study group
DOI：
10.1016/s0140-6736(03)13967-0
发表时间：
2003-07-26
期刊：
Lancet (London, England)
影响因子：
--
作者：
Kuiken T;Fouchier RA;Schutten M;Rimmelzwaan GF;van Amerongen G;van Riel D;Laman JD;de Jong T;van Doornum G;Lim W;Ling AE;Chan PK;Tam JS;Zambon MC;Gopal R;Drosten C;van der Werf S;Escriou N;Manuguerra JC;Stöhr K;Peiris JS;Osterhaus AD
通讯作者：
Osterhaus AD
影响因子：
56.9
作者：
Lipsitch, M;Cohen, T;Murray, M
通讯作者：
Murray, M
DOI：
10.1016/s0140-6736(03)13168-6
发表时间：
2003-05-03
期刊：
Lancet (London, England)
影响因子：
--
作者：
Seto WH;Tsang D;Yung RW;Ching TY;Ng TK;Ho M;Ho LM;Peiris JS;Advisors of Expert SARS group of Hospital Authority
通讯作者：
Advisors of Expert SARS group of Hospital Authority
DOI：
10.1016/s0140-6736(03)13413-7
发表时间：
2003-05-24
期刊：
Lancet (London, England)
影响因子：
--
作者：
Nicholls JM;Poon LL;Lee KC;Ng WF;Lai ST;Leung CY;Chu CM;Hui PK;Mak KL;Lim W;Yan KW;Chan KH;Tsang NC;Guan Y;Yuen KY;Peiris JS
通讯作者：
Peiris JS