Reprogramming of the Hevea brasiliensis Epigenome and Transcriptome in Response to Cold Stress.
Reprogramming of the Hevea brasiliensis Epigenome and Transcriptome in Response to Cold Stress.
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巴西橡胶表观基因组和转录组的重编程响应冷应激
DOI：
10.3389/fpls.2022.831839
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发表时间：
2022
影响因子：
5.6
通讯作者：
Huang X
中科院分区：
文献类型：
--
作者：
Tang X;Zhang Y;Yuan HM;Zhai J;Huang X
低温是限制橡胶种植园向高纬度地区推广的关键因素。以往的研究表明，冷诱导的DNA去甲基化与橡胶树冷反应基因（COR）的表达是协调的。本研究采用亚硫酸氢盐还原法对H.结果表明，冷处理诱导了全基因组DNA去甲基化，并改变了甲基化胞嘧啶的序列背景，但转座因子中的mCG甲基化水平略有提高。DNA甲基化组和转录组的综合分析揭示了400个基因的表达与DNA甲基化改变相关。基因上游区域的去甲基化与基因的高表达相关，而基因体区域的去甲基化与基因的高表达相关性较小。我们的研究结果表明，冷处理在全球范围内改变了橡胶树的基因组DNA甲基化状态，这可能会协调转录组的重编程。
Low temperature is a key factor limiting the rubber plantation extending to high latitude area. Previous work has shown that cold-induced DNA demethylation was coordinated with the expression of cold-responsive (COR) genes in Hevea brasiliensis. In this work, reduced representation bisulphite sequencing analysis of H. brasiliensis showed that cold treatment induced global genomic DNA demethylation and altered the sequence contexts of methylated cytosines, but the levels of mCG methylation in transposable elements were slightly enhanced by cold treatment. Integrated analysis of the DNA methylome and transcriptome revealed 400 genes whose expression correlated with altered DNA methylation. DNA demethylation in the upstream region of gene seems to correlate with higher gene expression, whereas demethylation in the gene body has less association. Our results suggest that cold treatment globally change the genomic DNA methylation status of the rubber tree, which might coordinate reprogramming of the transcriptome.
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影响因子：
12.3
作者：
Akalin A;Kormaksson M;Li S;Garrett-Bakelman FE;Figueroa ME;Melnick A;Mason CE
通讯作者：
Mason CE
影响因子：
4
作者：
Mai, Jing;Herbette, Stephane;Roeckel-Drevet, Patricia
通讯作者：
Roeckel-Drevet, Patricia
影响因子：
7.2
作者：
Mayr, Christine
通讯作者：
Mayr, Christine
DOI：
10.1038/nrg2719
发表时间：
2010-03
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.4
作者：
Duan C;Argout X;Gébelin V;Summo M;Dufayard JF;Leclercq J;Kuswanhadi;Piyatrakul P;Pirrello J;Rio M;Champion A;Montoro P
通讯作者：
Montoro P