Plasma Metabolite Profiles Following Consumption of Animal Protein and Soybean-Based Diet in Hypercholesterolemic Postmenopausal Women.
Plasma Metabolite Profiles Following Consumption of Animal Protein and Soybean-Based Diet in Hypercholesterolemic Postmenopausal Women.
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DOI：
10.3390/metabo12030209
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发表时间：
2022-02-25
期刊：
影响因子：
4.1
通讯作者：
Lichtenstein AH
中科院分区：
文献类型：
--
作者：
Huang NK;Matthan NR;Matuszek G;Lichtenstein AH
食物摄入量的主观报告可能不可靠。没有客观的方法可以区分不同蛋白质类型的饮食。为了解决这一差距，进行了随机对照交叉喂养试验的次要分析。评估空腹血浆代谢物谱及其与心脏代谢危险因素（CMRF）的相关性。高胆固醇血症绝经后妇女（N = 11）提供的饮食主要含有动物蛋白（AP）和大豆蛋白（SP）。非靶向代谢组学用于确定每个饮食阶段结束时的血浆代谢产物谱。使用针对错误发现率、偏最小二乘判别分析（PLS-DA）和受试者工作特征（ROC）调整的配对t检验比较了已鉴定代谢物（N = 829）的浓度。在鉴定的代谢物中，58种在AP和SP饮食之间存在显著差异;大多数是磷脂（n = 36），然后是氨基酸（n = 10），异生物质（n = 7），维生素/维生素相关（n = 3）和脂质（n = 2）。在前10种代谢物中，氨基酸衍生的代谢物、磷脂和外源性物质构成了因膳食蛋白质类型而不同的主要类别。ROC曲线证实前10种代谢物是AP和SP丰富饮食的潜在鉴别生物标志物。总之，氨基酸衍生的代谢物、磷脂酰乙醇胺衍生的代谢物和胆甾酮被确定为区分膳食蛋白类型的潜在代谢物生物标志物。
Subjective reporting of food intake can be unreliable. No objective method is available to distinguish between diets differing in protein type. To address this gap, a secondary analysis of a randomized controlled cross-over feeding trial was conducted. Assessed were fasting plasma metabolite profiles and their associations with cardiometabolic risk factors (CMRFs). Hypercholesterolemic post-menopausal women (N = 11) were provided with diets containing predominantly animal protein (AP) and soy protein (SP). Untargeted metabolomics were used to determine the plasma metabolite profiles at the end of each diet phase. Concentrations of identified metabolites (N = 829) were compared using paired t-tests adjusted for false discovery rate, partial least square-discrimination analysis (PLS-DA) and receiver operating characteristics (ROC). Among the identified metabolites, 58 differed significantly between the AP and SP diets; the majority were phospholipids (n = 36), then amino acids (n = 10), xenobiotics (n = 7), vitamin/vitamin-related (n = 3) and lipids (n = 2). Of the top 10 metabolites, amino acid-derived metabolites, phospholipids and xenobiotics comprised the main categories differing due to dietary protein type. ROC curves confirmed that the top 10 metabolites were potential discriminating biomarkers for AP- and SP-rich diets. In conclusion, amino acid-derived metabolites, phosphatidylethanolamine-derived metabolites and isoflavones were identified as potential metabolite biomarkers distinguishing between dietary protein type.
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影响因子：
6.5
作者：
Singh, Ratnesh Kumar;Fullerton, Morgan D.;Bakovic, Marica
通讯作者：
Bakovic, Marica
影响因子：
4.4
作者：
Andersen, Maj-Britt S.;Rinnan, Asmund;Dragsted, Lars O.
通讯作者：
Dragsted, Lars O.
影响因子：
4.8
作者：
Leonardi, Roberta;Frank, Matthew W.;Jackowski, Suzanne
通讯作者：
Jackowski, Suzanne
影响因子：
5
作者：
McKeown, Nicola M.;Marklund, Matti;Chen, C-Y. Oliver
通讯作者：
Chen, C-Y. Oliver
DOI：
10.3945/ajcn.115.111989
发表时间：
2015-12
期刊：
The American journal of clinical nutrition
影响因子：
--
作者：
Schmidt JA;Rinaldi S;Ferrari P;Carayol M;Achaintre D;Scalbert A;Cross AJ;Gunter MJ;Fensom GK;Appleby PN;Key TJ;Travis RC
通讯作者：
Travis RC