Reduction of stearoyl-CoA desaturase (SCD) contributes muscle atrophy through the excess endoplasmic reticulum stress in chronic kidney disease.
Reduction of stearoyl-CoA desaturase (SCD) contributes muscle atrophy through the excess endoplasmic reticulum stress in chronic kidney disease.
复制标题
降低甲酰基-COA去饱和酶（SCD）通过慢性肾脏疾病中过量的内质网应激造成肌肉萎缩。
DOI：
10.3164/jcbn.20-24
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发表时间：
2020-09
影响因子：
2.4
通讯作者：
Taketani Y
中科院分区：
文献类型：
--
作者：
Niida Y;Masuda M;Adachi Y;Yoshizawa A;Ohminami H;Mori Y;Ohnishi K;Yamanaka-Okumura H;Uchida T;Nikawa T;Yamamoto H;Miyazaki M;Taketani Y
骨骼肌萎缩与慢性肾脏病（CKD）患者的死亡率和预后不良相关。然而，CKD导致肌肉萎缩的潜在机制尚未完全了解。脂质的质量（lipquality），其被定义为不同脂质种类的功能特征，最近被认为是各种疾病的病理学。在这项研究中，我们研究了硬脂酰辅酶A去饱和酶（SCD）的作用，它催化饱和脂肪酸转化为单不饱和脂肪酸，在骨骼肌肌肌萎缩的CKD模型动物。与对照组相比，CKD大鼠萎缩的腓肠肌中SCD活性及其基因表达降低。其次，油酸阻断SCD抑制剂引起的C2C12肌管厚度的减少和内质网应激的增加。此外，内质网应激抑制剂还能改善CKD诱导的小鼠肌肉萎缩（握力减弱和腓肠肌肌纤维尺寸减小）和SCD抑制剂引起的C2C12肌管厚度减少。这些结果表明，SCD活性的抑制通过CKD中的过度内质网应激引起肌肉萎缩。
Skeletal muscle atrophy is associated with mortality and poor prognosis in patients with chronic kidney disease (CKD). However, underlying mechanism by which CKD causes muscle atrophy has not been completely understood. The quality of lipids (lipoquality), which is defined as the functional features of diverse lipid species, has recently been recognized as the pathology of various diseases. In this study, we investigated the roles of the stearoyl-CoA desaturase (SCD), which catalyzes the conversion of saturated fatty acids into monounsaturated fatty acids, in skeletal muscle on muscle atrophy in CKD model animals. In comparison to control rats, CKD rats decreased the SCD activity and its gene expression in atrophic gastrocnemius muscle. Next, oleic acid blocked the reduction of the thickness of C2C12 myotubes and the increase of the endoplasmic reticulum stress induced by SCD inhibitor. Furthermore, endoplasmic reticulum stress inhibitor ameliorated CKD-induced muscle atrophy (the weakness of grip strength and the decrease of muscle fiber size of gastrocnemius muscle) in mice and the reduction of the thickness of C2C12 myotubes by SCD inhibitor. These results suggest that the repression of SCD activity causes muscle atrophy through excessive endoplasmic reticulum stress in CKD.
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影响因子：
2.4
作者：
Imi Y;Yabiki N;Abuduli M;Masuda M;Yamanaka-Okumura H;Taketani Y
通讯作者：
Taketani Y
影响因子：
2.4
作者：
Okamura, Takuro;Hashimoto, Yoshitaka;Fukui, Michiaki
通讯作者：
Fukui, Michiaki
影响因子：
4.8
作者：
Lecker, SH;Jagoe, RT;Goldberg, AL
通讯作者：
Goldberg, AL
影响因子：
8.9
作者：
Aniort, Julien;Stella, Alexandre;Taillandier, Daniel
通讯作者：
Taillandier, Daniel
影响因子：
3.7
作者：
Lee, Dustin M.;Sevits, Kyle J.;Gentile, Christopher L.
通讯作者：
Gentile, Christopher L.