Diversity of the Killer Cell Ig-Like Receptors of Rhesus Monkeys1 2
Diversity of the Killer Cell Ig-Like Receptors of Rhesus Monkeys1 2
复制标题
恒河猴杀伤细胞 Ig 样受体的多样性1 2
作者：
K. Hershberger;Richa Shyam;Ayako Miura;N. Letvin
由于杀伤细胞免疫球蛋白样受体（KIR）仅在人类和黑猩猩中被表征，因此我们对其进化历史没有充分的了解。因此，对编码5只恒河猴的KIR分子的cDNA进行了表征，发现其与在人类和黑猩猩中鉴定的KIR分子不同。虽然在人类和黑猩猩中只检测到一种KIR2DL4分子，但在猴子中鉴定出两种不同的KIR2DL4同源物。虽然两种人KIR3DL分子的多态性有限，但猴子中的KIR3DL同源物具有高度多态性。在研究的每只猴子中鉴定了多达5种KIR3DL同源物，并且在5只恒河猴中检测到11种不同的KIR3DL分子。在恒河猴中鉴定了两个新的KIR分子家族，KIR3DH和KIR1D。KIR 3 DH分子具有三个IG结构域、与含有精氨酸的KIR 2 DL 4分子同源的跨膜结构域和短胞质结构域。具有这些特征，KIR3DH分子类似于人KIR分子的活化形式。KIR 1D分子在第二个IG结构域发生移码之前仅编码一个完整的IG结构域，导致分子的提前终止。存在编码至少一个IG结构域以及跨膜和胞质结构域的KIR 1D的多种剪接变体。恒河猴KIR3DL同源物和新的KIR3DH和KIR1D分子的广泛多样性表明，KIR家族分子在灵长类动物的进化过程中迅速进化。
Because the killer cell Ig-like receptors (KIRs) have only been characterized in humans and chimpanzees, we do not have a full understanding of their evolutionary history. Therefore, cDNAs encoding the KIR molecules of five rhesus monkeys were characterized, and were found to differ from the KIR molecules identified in humans and chimpanzees. Whereas only one KIR2DL4 molecule is detected in humans and chimpanzees, two distinct KIR2DL4 homologues were identified in the monkeys. Although the two human KIR3DL molecules are limited in their polymorphism, the KIR3DL homologues in the monkeys were highly polymorphic. Up to five KIR3DL homologues were identified in each monkey that was studied, and eleven distinct KIR3DL molecules were detected in the five rhesus monkeys. Two novel families of KIR molecules were identified in the rhesus monkeys, KIR3DH and KIR1D. The KIR3DH molecules have three Ig domains, transmembrane domains homologous to KIR2DL4 molecules that contain an arginine, and short cytoplasmic domains. With these features, the KIR3DH molecules resemble the activating forms of the human KIR molecules. The KIR1D molecule encodes only one complete Ig domain before a frame-shift in the second Ig domain occurs, leading to early termination of the molecule. Multiple splice variants of KIR1D exist that encode at least one Ig domain, as well as transmembrane and cytoplasmic domains. The extensive diversity of the rhesus monkey KIR3DL homologues and the novel KIR3DH and KIR1D molecules suggests that the KIR family of molecules has evolved rapidly during the evolution of primates.
登录
查看更多内容
影响因子：
32.4
作者：
Khakoo, SI;Rajalingam, R;Parham, P
通讯作者：
Parham, P
影响因子：
--
作者：
A. Selvakumar;U. Steffens;Bo Dupont
通讯作者：
A. Selvakumar;U. Steffens;Bo Dupont
影响因子：
32.4
作者：
Uhrberg, M;Valiante, NM;Parham, P
通讯作者：
Parham, P
NK 细胞抑制性受体的直接结合和功能转移揭示了 HLA-C 同种异型识别的新模式。
DOI：
--
发表时间：
1998
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Winter,CC;Gumperz,JE;Parham,P;Long,EO;Wagtmann,N
通讯作者：
Wagtmann,N
MHC-E 位点是所有已知灵长类 I 类组织相容性基因中最保守的。
DOI：
--
发表时间：
1998
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Knapp,LA;Cadavid,LF;Watkins,DI
通讯作者：
Watkins,DI