Fluorescent glycosylamides produced by microscale derivatization of free glycans for natural glycan microarrays.
Fluorescent glycosylamides produced by microscale derivatization of free glycans for natural glycan microarrays.
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DOI：
10.1021/cb900067h
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发表时间：
2009-09-18
影响因子：
4
通讯作者：
Cummings, Richard D.
中科院分区：
文献类型：
--
作者：
Song, Xuezheng;Lasanajak, Yi;Xia, Baoyun;Smith, David F.;Cummings, Richard D.
已经开发了一种用于创建天然来源的聚糖微阵列的新策略。糖基胺由游离还原聚糖制备，并通过与丙烯酰氯反应以生成糖基酰胺来稳定，其中还原单糖具有闭环结构。被保护的聚糖的臭氧分解产生活性醛，通过还原胺化将双官能荧光连接体偶联至该活性醛。荧光衍生物通过残留的伯烷基胺容易地偶联以产生聚糖微阵列。这种策略保留了抗体识别所需的聚糖的结构特征，并允许开发荧光聚糖的天然阵列，其中保留了还原末端糖残基的环状吡喃糖结构。
A novel strategy for creating naturally-derived glycan microarrays has been developed. Glycosylamines are prepared from free reducing glycans and stabilized by reaction with acryloyl chloride to generate a glycosylamide in which the reducing monosaccharide has a closed ring structure. Ozonolysis of the protected glycan yields an active aldehyde, to which a bifunctional fluorescent linker is coupled by reductive amination. The fluorescent derivatives are easily coupled through a residual primary alkylamine to generate glycan microarrays. This strategy preserves structural features of glycans required for antibody recognition, and allows development of natural arrays of fluorescent glycans in which the cyclic pyranose structure of the reducing-end sugar residue is retained.
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影响因子：
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通讯作者：
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