De novo genome assembly of the soil-borne fungus and tomato pathogen Pyrenochaeta lycopersici.
De novo genome assembly of the soil-borne fungus and tomato pathogen Pyrenochaeta lycopersici.
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DOI：
10.1186/1471-2164-15-313
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发表时间：
2014-04-27
期刊：
影响因子：
4.4
通讯作者：
Delledonne M
中科院分区：
文献类型：
--
作者：
Aragona M;Minio A;Ferrarini A;Valente MT;Bagnaresi P;Orrù L;Tononi P;Zamperin G;Infantino A;Valè G;Cattivelli L;Delledonne M
番茄核壳菌（Pyrenochaeta lycopersici）是一种土壤中的子囊菌病原体，可引起番茄（Solanum lycopersicum）和其他茄科作物的木栓性根腐病，使水果产量减少高达75%。尽管真菌病原体对植物生长和果实产量有显著影响，但感染根部的真菌病原体受到的关注少于感染作物气生部分的真菌病原体。我们基于Illumina短读段组装了54.9Mb的番茄疫霉基因组草图序列，并注释了大约17，000个基因。番茄疫霉基因组与半营养型和坏死营养型密切相关，与真菌的表型特征及其生活方式一致。与宿主-病原体相互作用有关的几个基因家族有很强的代表性，包括负责营养吸收、杀真菌剂解毒和植物细胞壁降解的基因家族，后者证实了基因组的大部分致力于真菌的致病活性。我们没有发现MAT基因，这与番茄疫霉作为不完全真菌的分类是一致的，但是我们观察到与异核体不相容性（HI）相关的基因家族的显著扩展。番茄疫霉基因组序列草图提供了对真菌生活方式的分子和遗传基础的深入了解，描述了以前未知的致病行为，并定义了允许这种无性真菌增加遗传多样性并获得新的致病性状的策略。
Pyrenochaeta lycopersici is a soil-dwelling ascomycete pathogen that causes corky root rot disease in tomato (Solanum lycopersicum) and other Solanaceous crops, reducing fruit yields by up to 75%. Fungal pathogens that infect roots receive less attention than those infecting the aerial parts of crops despite their significant impact on plant growth and fruit production. We assembled a 54.9Mb P. lycopersici draft genome sequence based on Illumina short reads, and annotated approximately 17,000 genes. The P. lycopersici genome is closely related to hemibiotrophs and necrotrophs, in agreement with the phenotypic characteristics of the fungus and its lifestyle. Several gene families related to host–pathogen interactions are strongly represented, including those responsible for nutrient absorption, the detoxification of fungicides and plant cell wall degradation, the latter confirming that much of the genome is devoted to the pathogenic activity of the fungus. We did not find a MAT gene, which is consistent with the classification of P. lycopersici as an imperfect fungus, but we observed a significant expansion of the gene families associated with heterokaryon incompatibility (HI). The P. lycopersici draft genome sequence provided insight into the molecular and genetic basis of the fungal lifestyle, characterizing previously unknown pathogenic behaviors and defining strategies that allow this asexual fungus to increase genetic diversity and to acquire new pathogenic traits.
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影响因子：
3.5
作者：
Carlile, AJ;Bindschedler, LV;Cooper, RM
通讯作者：
Cooper, RM
影响因子：
1.4
作者：
Bayraktar, Harun;Oksal, Ercin
通讯作者：
Oksal, Ercin
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
2.8
作者：
de Gruyter, Johannes;Woudenberg, Joyce H. C.;Crous, Pedro W.
通讯作者：
Crous, Pedro W.
影响因子：
2.5
作者：
Flores, A;Chet, I;HerreraEstrella, A
通讯作者：
HerreraEstrella, A