Replication of Epstein-Barr virus primary infection in human tonsil tissue explants.
Replication of Epstein-Barr virus primary infection in human tonsil tissue explants.
复制标题
人类扁桃体组织外植体中爱泼斯坦 - 巴尔病毒原发性感染的复制。
DOI：
10.1371/journal.pone.0025490
复制
发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Kimura H
中科院分区：
文献类型：
--
作者：
Gotoh K;Ito Y;Maruo S;Takada K;Mizuno T;Teranishi M;Nakata S;Nakashima T;Iwata S;Goshima F;Nakamura S;Kimura H
EB病毒（EBV）可引起多种病毒相关疾病，但尚未开发出针对该病毒的抗病毒剂。因此，动物模型对于EBV相关感染的病理分析和治疗方法的阐明是必不可少的。为了建立研究EB病毒感染的模型系统，我们检测了B 95 -8病毒和表达增强型绿色荧光蛋白（EGFP）的重组EB病毒在人淋巴组织中复制的能力。将在常规扁桃体切除术中手术切除的人扁桃体组织切成小块，并置于空气界面处培养基中的胶原海绵凝胶顶部，然后将无细胞病毒悬液直接应用于每个组织块的顶部。在用B 95 -8和EGFP-EBV感染的组织模型中，在感染后12-15天至24天观察到培养基中EBV DNA水平增加。在培养过程中，从培养基中收集的细胞中的8个EBV相关基因的表达水平增加。在石蜡包埋切片中，在滤泡间区域检测到EBV编码的小RNA阳性细胞。流式细胞术分析显示，大多数EGFP+细胞是CD 3 − CD 56 − CD 19 + HLA-DR+，代表幼稚（免疫球蛋白D+）和记忆（CD 27+）B细胞。此外，在这个模型中，EBV复制抑制阿昔洛韦治疗剂量依赖性的方式。这些数据表明，该模型具有潜在的用于在原发感染时的局部组织的病理分析，以及用于筛选新的抗病毒剂。
Epstein-Barr virus (EBV) may cause a variety of virus-associated diseases, but no antiviral agents have yet been developed against this virus. Animal models are thus indispensable for the pathological analysis of EBV-related infections and the elucidation of therapeutic methods. To establish a model system for the study of EBV infection, we tested the ability of B95–8 virus and recombinant EBV expressing enhanced green fluorescent protein (EGFP) to replicate in human lymphoid tissue. Human tonsil tissues that had been surgically removed during routine tonsillectomy were sectioned into small blocks and placed on top of collagen sponge gels in culture medium at the air-interface, then a cell-free viral suspension was directly applied to the top of each tissue block. Increasing levels of EBV DNA in culture medium were observed after 12–15 days through 24 days post-infection in tissue models infected with B95–8 and EGFP-EBV. Expression levels of eight EBV-associated genes in cells collected from culture medium were increased during culture. EBV-encoded small RNA-positive cells were detected in the interfollicular areas in paraffin-embedded sections. Flow cytometric analyses revealed that most EGFP+ cells were CD3− CD56− CD19+ HLA-DR+, and represented both naïve (immunoglobulin D+) and memory (CD27+) B cells. Moreover, EBV replication in this model was suppressed by acyclovir treatment in a dose-dependent manner. These data suggest that this model has potential for use in the pathological analysis of local tissues at the time of primary infection, as well as for screening novel antiviral agents.
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影响因子：
30.3
作者：
Lisco, Andrea;Vanpouille, Christophe;Margolis, Leonid
通讯作者：
Margolis, Leonid
影响因子：
82.9
作者：
Glushakova, S;Grivel, JC;Margolis, LB
通讯作者：
Margolis, LB
影响因子：
32.4
作者：
Kurth, J;Spieker, T;Küppers, R
通讯作者：
Küppers, R
影响因子：
3.8
作者：
Iwata, Seiko;Wada, Kaoru;Kimura, Hiroshi
通讯作者：
Kimura, Hiroshi
影响因子：
5.4
作者：
Grivel, JC;Santoro, F;Lusso, P
通讯作者：
Lusso, P