Multi-Toxin Resistance Enables Pink Bollworm Survival on Pyramided Bt Cotton.
Multi-Toxin Resistance Enables Pink Bollworm Survival on Pyramided Bt Cotton.
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多毒素耐药性使金字化BT棉花上的粉红色螺丝虫存活。
DOI：
10.1038/srep16554
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发表时间：
2015-11-12
影响因子：
4.6
通讯作者：
Tabashnik BE
中科院分区：
文献类型：
--
作者：
Fabrick JA;Unnithan GC;Yelich AJ;DeGain B;Masson L;Zhang J;Carrière Y;Tabashnik BE
转基因作物生产苏云金杆菌（Bt）蛋白杀死主要害虫，提供经济和环境效益。然而，害虫抗性的进化威胁着这种Bt作物的持续成功。为了延缓或对抗抗性，产生两种或多种Bt蛋白的转基因植物“金字塔”已经被广泛采用，这些蛋白可以杀死同一种害虫。在美国，红铃虫（棉红铃虫）的田间种群仍然对Bt毒素Cry1Ac和Cry2Ab敏感，但在印度，田间进化的对产生Cry1Ac的Bt棉花的实际抗性已经广泛发生。在这里，我们使用了两轮实验室选择，使红铃虫对Cry 2Ab的抗性提高了18，000至150，000倍。对Cry2Ab的抗性遗传是隐性的，常染色体的，主要由一个位点赋予，并且独立于Cry1Ac抗性。我们通过将Cry2Ab抗性菌株与Cry1Ac抗性菌株杂交，然后用Cry2Ab进行一次选择，创建了对两种毒素都具有高抗性的菌株。这种多毒素抗性菌株存活在现场收集的Bt棉铃生产这两种毒素。这里的结果证明了对Bt植物抗性进化的风险，特别是当毒素被顺序部署并且避难所稀缺时，如印度的Bt棉花和粉红棉铃虫。
Transgenic crops producing Bacillus thuringiensis (Bt) proteins kill key insect pests, providing economic and environmental benefits. However, the evolution of pest resistance threatens the continued success of such Bt crops. To delay or counter resistance, transgenic plant “pyramids” producing two or more Bt proteins that kill the same pest have been adopted extensively. Field populations of the pink bollworm (Pectinophora gossypiella) in the United States have remained susceptible to Bt toxins Cry1Ac and Cry2Ab, but field-evolved practical resistance to Bt cotton producing Cry1Ac has occurred widely in India. Here we used two rounds of laboratory selection to achieve 18,000- to 150,000-fold resistance to Cry2Ab in pink bollworm. Inheritance of resistance to Cry2Ab was recessive, autosomal, conferred primarily by one locus, and independent of Cry1Ac resistance. We created a strain with high resistance to both toxins by crossing the Cry2Ab-resistant strain with a Cry1Ac-resistant strain, followed by one selection with Cry2Ab. This multi-toxin resistant strain survived on field-collected Bt cotton bolls producing both toxins. The results here demonstrate the risk of evolution of resistance to pyramided Bt plants, particularly when toxins are deployed sequentially and refuges are scarce, as seen with Bt cotton and pink bollworm in India.
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影响因子：
3.8
作者：
Bravo, Alejandra;Likitvivatanavong, Supaporn;Gill, Sarjeet S.;Soberon, Mario
通讯作者：
Soberon, Mario
影响因子：
4.6
作者：
Brévault T;Tabashnik BE;Carrière Y
通讯作者：
Carrière Y
DOI：
10.1023/a:1009673622862
发表时间：
1998-01-01
期刊：
Integrated Pest Management Reviews
影响因子：
--
作者：
Henneberry, T. J.;Naranjo, S. E.
通讯作者：
Naranjo, S. E.
影响因子：
1.7
作者：
GOULD, F
通讯作者：
GOULD, F
DOI：
10.1098/rspb.2003.2497
发表时间：
2003-11-07
影响因子：
4.7
作者：
Janmaat, AF;Myers, J
通讯作者：
Myers, J