Crystallographic texture and mineral concentration quantification of developing and mature human incisal enamel.
Crystallographic texture and mineral concentration quantification of developing and mature human incisal enamel.
复制标题
DOI：
10.1038/s41598-018-32425-y
复制
发表时间：
2018-09-27
影响因子：
4.6
通讯作者：
Al-Jawad M
中科院分区：
文献类型：
--
作者：
Al-Mosawi M;Davis GR;Bushby A;Montgomery J;Beaumont J;Al-Jawad M
对于人类牙釉质，精确的矿化进程在空间上是什么，矿化的精确时间是什么？这在对基质介导的生物矿化事件的基本理解中是一个重要的问题，特别是因为我们可以利用我们对人类这种自然组织生长的理解来开发仿生方法来修复和替换丢失的釉质组织。了解人体组织尤其重要，因为不同物种的矿化在空间和时间上都有相当不同的进程。本研究利用同步辐射X射线衍射仪和X射线显微断层扫描技术，对5颗不同釉质成熟/矿化阶段的人中切牙进行了空间定位。从最早的发育阶段开始，两个微晶取向的种群共存，大约40°的微晶种群之间的角度间隔随着牙冠内位置的不同而变化。总体而言，一个群体的织构幅度显著较低，对整体晶体结构的贡献百分比较高，而另一个群体的织构幅度仅为20-30%，但具有显著较高的织构幅度。这一定量分析使我们能够了解人牙釉质中两组微晶之间的复杂和协同的结构-功能关系。随着成熟时间的延长，牙釉质-牙本质交界处周围和颈部切尖的矿物质浓度增加。背散射电子定量分析表明棱柱核的成矿作用先于棱柱边界的成矿作用。这些结果为精确理解人牙釉质的自然生长提供了新的见解。
For human dental enamel, what is the precise mineralization progression spatially and the precise timing of mineralization? This is an important question in the fundamental understanding of matrix-mediated biomineralization events, but in particular because we can use our understanding of this natural tissue growth in humans to develop biomimetic approaches to repair and replace lost enamel tissue. It is important to understand human tissues in particular since different species have quite distinct spatial and temporal progression of mineralization. In this study, five human central incisors at different stages of enamel maturation/mineralization were spatially mapped using synchrotron X-ray diffraction and X-ray microtomography techniques. From the earliest developmental stage, two crystallite-orientation populations coexist with angular separations between the crystallite populations of approximately 40° varying as a function of position within the tooth crown. In general, one population had significantly lower texture magnitude and contributed a higher percentage to the overall crystalline structure, compared to the other population which contributed only 20–30% but had significantly higher texture magnitude. This quantitative analysis allows us to understand the complex and co-operative structure-function relationship between two populations of crystallites within human enamel. There was an increase in the mineral concentration from the enamel-dentin junction peripherally and from the incisal tip cervically as a function of maturation time. Quantitative backscattered-electron analyses showed that mineralization of prism cores precedes that of prism boundaries. These results provide new insights into the precise understanding of the natural growth of human enamel.
登录
查看更多内容
影响因子：
3
作者：
DEUTSCH, D;GEDALIA, I
通讯作者：
GEDALIA, I
影响因子：
3
作者：
Beniash, Elia;Metzler, Rebecca A.;Lam, Raymond S. K.;Gilbert, P. U. P. A.
通讯作者：
Gilbert, P. U. P. A.
影响因子：
3
作者：
CRABB, HSM;DARLING, AI
通讯作者：
DARLING, AI
影响因子：
4.4
作者：
Davis, Graham R.;Evershed, Anthony N. Z.;Mills, David
通讯作者：
Mills, David
影响因子：
7.6
作者：
AVERY, JK;KNAPP, DE;VISSER, RL
通讯作者：
VISSER, RL