JAK/STAT signaling is required for hinge growth and patterning in the Drosophila wing disc.
JAK/STAT signaling is required for hinge growth and patterning in the Drosophila wing disc.
复制标题
DOI：
10.1016/j.ydbio.2013.08.016
复制
发表时间：
2013-10-15
影响因子：
2.7
通讯作者：
Bach, Erika A.
中科院分区：
文献类型：
--
作者：
Ayala-Camargo, Aidee;Anderson, Abigail M.;Amoyel, Marc;Rodrigues, Aloma B.;Flaherty, Maria Sol;Bach, Erika A.
JAK/STAT 信号传导位于机翼铰链，但其功能尚不清楚。在这里，我们表明果蝇 STAT Stat92E 位于同胸的下游，并且是通过细胞自主调节铰链特异性因子来实现铰链发育所必需的。在铰链内，Stat92E 活性仅限于缺乏 Nubbin 和 Teashirt 的间隙域细胞。虽然缺乏 Stat92E 的间隙域细胞的增殖显着降低，但 JAK/STAT 信号传导的增加不会扩展该域。因此，该途径对于间隙域的生长是必要的但还不够。我们发现，减少的 Wingless (Wg) 信号主要抑制铰链中 Stat92E 的活性。然而，异位 JAK/STAT 信号传导不会扰乱铰链中的 Wg 表达。我们报告了 Stat92E 和 notum 因子 Araucan 之间的负相互作用，导致来自 notum 的 JAK/STAT 信号传导受到限制。此外，我们发现远端因子 Nub 抑制未配对的配体以及 Stat92E 活性。这些数据表明 JAK/STAT 信号传导的远端扩展对翼片发育有害。事实上，假定翼片中“不配对”的错误表达会导致成虫翅膀小而发育不良。我们得出的结论是，JAK/STAT 信号对于铰链命运规范和间隙结构域的生长至关重要，并且其对铰链的限制是正确的翼发育所必需的。
JAK/STAT signaling is localized to the wing hinge, but its function is not known. Here we show that the Drosophila STAT Stat92E is downstream of Homothorax and is required for hinge development by cell-autonomously regulating hinge-specific factors. Within the hinge, Stat92E activity becomes restricted to gap domain cells that lack Nubbin and Teashirt. While gap domain cells lacking Stat92E have significantly reduced proliferation, increased JAK/STAT signaling there does not expand this domain. Thus, this pathway is necessary but not sufficient for gap domain growth. We show that reduced Wingless (Wg) signaling dominantly inhibits Stat92E activity in the hinge. However, ectopic JAK/STAT signaling does not perturbs Wg expression in the hinge. We report negative interactions between Stat92E and the notum factor Araucan, resulting in restriction of JAK/STAT signaling from the notum. In addition, we find that the distal factor Nub represses the ligand unpaired as well as Stat92E activity. These data suggest that distal expansion of JAK/STAT signaling is deleterious to wing blade development. Indeed, mis-expression of Unpaired within the presumptive wing blade causes small, stunted adult wings. We conclude that JAK/STAT signaling is critical for hinge fate specification and growth of the gap domain and that its restriction to the hinge is required for proper wing development.
登录
查看更多内容
影响因子：
1.2
作者：
Bach, Erika A.;Ekas, Laura A.;Baeg, Gyeong-Hun
通讯作者：
Baeg, Gyeong-Hun
影响因子：
2.7
作者：
Hombría, JCG;Brown, S;Zeidler, MP
通讯作者：
Zeidler, MP
DOI：
10.1073/pnas.94.21.11405
发表时间：
1997-10-14
影响因子：
11.1
作者：
Cifuentes, FJ;GarciaBellido, A
通讯作者：
GarciaBellido, A
影响因子：
2.7
作者：
Ekas LA;Cardozo TJ;Flaherty MS;McMillan EA;Gonsalves FC;Bach EA
通讯作者：
Bach EA
影响因子：
9.2
作者：
Lovegrove, Bridget;Simoes, Sergio;Castelli-Gair Hombria, James
通讯作者：
Castelli-Gair Hombria, James