Fluorapatite and fluorohydroxyapatite apatite surfaces drive adipose-derived stem cells to an osteogenic lineage.
Fluorapatite and fluorohydroxyapatite apatite surfaces drive adipose-derived stem cells to an osteogenic lineage.
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氟磷灰石和氟羟基磷灰石表面驱动脂肪干细胞形成成骨谱系。
DOI：
10.1016/j.jmbbm.2021.104950
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发表时间：
2022
影响因子：
3.9
通讯作者：
Shea,Jill
中科院分区：
文献类型：
--
作者：
Jeyapalina,Sujee;Hillas,Elaine;Beck,JamesPeter;Agarwal,Jayant;Shea,Jill
目的羟基磷灰石（Hydroxyapatite，HA）支架材料是临床治疗严重骨缺损的常用替代材料。本研究旨在研究羟基磷灰石、氟磷灰石（FA）和氟羟基磷灰石（FHA）的氟化衍生物与脂肪源性干细胞（ADSC）联合作为骨支架的潜在益处。如果FHA和FA表面可以驱动干细胞分化为成骨表型，则这些陶瓷支架与ADSC的组合可以产生具有与自体骨相当的机械强度和重塑潜力的材料。本研究旨在研究在不同烧结温度下产生的磷灰石表面HA、FA和FHA驱动ADSC向成骨谱系发展的能力。研究方法：将在1150 °C和1250 °C下烧结的HA、FHA和FA表面用ADSC接种，并在接种后2天和10天评价细胞生长以及成骨标志物的基因和蛋白质表达。结果：在体外，ADSC细胞在所有表面上都是活的;然而，这些细胞仅在磷灰石表面上观察到向成骨细胞谱系的分化。与HA和细胞培养对照相比，接种在FA和FHA上的ADSC在第2天更大程度地表达与成骨分化标志物相关的基因和蛋白。到第10天，与细胞培养对照相比，HA、FA和FHA均表达更多的骨分化标志物。结论：FA和FHA磷灰石支架较HA表面能更早地促进ADSCs的分化。将磷灰石支架与ADSCs结合具有改善骨损伤后骨再生的潜力。
PurposeHydroxyapatite (HA) scaffolds are common replacement materials used in the clinical management of critical-sized bone defects. This study was undertaken to examine the potential benefits of fluoridated derivatives of hydroxyapatite, fluorapatite (FA), and fluorohydroxyapatite (FHA) as bone scaffolds in conjunction with adipose-derived stem cells (ADSCs). If FHA and FA surfaces could drive the differentiation of stem cells to an osteogenic phenotype, the combination of these ceramic scaffolds with ADSCs could produce materials with mechanical strength and remodeling potential comparable to autologous bone. This study was designed to investigate the ability of the apatite surfaces HA, FA, and FHA produced at different sintering temperatures to drive ADSCs toward osteogenic lineages. Methods: HA, FHA, and FA surfaces sintered at 1150 °C and 1250 °C were seeded with ADSCs and evaluated for cell growth and gene and protein expression of osteogenic markers at 2 and 10 days post-seeding. Results:In vitro,ADSC cells were viable on all surfaces; however, differentiation of these cells into osteoblastic lineage only observed in apatite surfaces. ADSCs seeded on FA and FHA expressed genes and proteins related to osteogenic differentiation markers to a greater extent by Day 2 when compared to HA and cell culture controls. By day 10, HA, FA, and FHA all expressed more bone differentiation markers compared to cell culture controls. Conclusion: FA and FHA apatite scaffolds may promote the differentiation of ADSCs at an earlier time point than HA surfaces. Combining apatite scaffolds with ADSCs has the potential to improve bone regeneration following bone injury.
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DOI：
10.1023/a:1008986430940
发表时间：
2001-02
期刊：
Journal of Materials Science: Materials in Medicine
影响因子：
--
作者：
N. Patel;I. Gibson;S. Ke;S. Best;W. Bonfield
通讯作者：
N. Patel;I. Gibson;S. Ke;S. Best;W. Bonfield
影响因子：
14
作者：
Liu, Jun;Wang, Xiaodong;Jin, Qiming;Jin, Taocong;Chang, Syweren;Zhang, Zhaocheng;Czajka-Jakubowska, Agata;Giannobile, William V.;Noer, Jacques E.;Clarkson, Brian H.
通讯作者：
Clarkson, Brian H.
影响因子：
14
作者：
Zhao, G.;Raines, A. L.;Boyan, B. D.
通讯作者：
Boyan, B. D.
影响因子：
4.1
作者：
Olivares-Navarrete, Rene;Hyzy, Sharon L.;Haithcock, David A.;Cundiff, Caitlin A.;Schwartz, Zvi;Boyan, Barbara D.
通讯作者：
Boyan, Barbara D.
影响因子：
14
作者：
Kim, HW;Lee, EJ;Knowles, JC
通讯作者：
Knowles, JC