The stimulation of adipose-derived stem cell differentiation and mineralization by ordered rod-like fluorapatite coatings.
The stimulation of adipose-derived stem cell differentiation and mineralization by ordered rod-like fluorapatite coatings.
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DOI：
10.1016/j.biomaterials.2012.03.050
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发表时间：
2012-07
期刊：
影响因子：
14
通讯作者：
Clarkson, Brian H.
中科院分区：
文献类型：
--
作者：
Liu, Jun;Wang, Xiaodong;Jin, Qiming;Jin, Taocong;Chang, Syweren;Zhang, Zhaocheng;Czajka-Jakubowska, Agata;Giannobile, William V.;Noer, Jacques E.;Clarkson, Brian H.
在本研究中，体外研究了金属盘的有序棒状 FA 涂层对脂肪干细胞（ASC）生长、分化和矿化的影响；及其体内矿物质的诱导作用。 3天和7天后，金属表面上的细胞数量明显高于有序和无序FA表面上的细胞数量。然而，4 周后，在有甚至没有成骨诱导的情况下，两个 FA 表面上诱导出更多的矿物质形成。成骨曲线显示，与金属表面相比，ASC 在 FA 表面生长第 7 天和第 21 天时，一组促成骨转录本和骨矿化表型标记物上调。除了 BMP 和 TGFβ 信号通路外，EGF 和 FGF 通路似乎也参与 ASC 的分化和矿化。体内研究表明，有序 FA 涂层可加速和增强矿化组织的形成。 5 周后，超过 80% 的订购 FA 涂层与覆盖种植体的矿化组织层融为一体。 FA 晶体的内在特性和 FA 涂层的形貌似乎主导着细胞分化和矿化过程。
In this study, the effect of ordered rod-like FA coatings of metal discs on adipose-derived stem cell (ASC)’s growth, differentiation and mineralization was studied in vitro; and their mineral inductive effects in vivo. After 3 and 7 days, the cell number on the metal surfaces was significantly higher than those on the ordered and disordered FA surfaces. However, after 4 weeks much greater amounts of mineral formation was induced on the two FA surfaces with and even without osteogenesis induction. The osteogenic profiles showed the up regulation of a set of pro-osteogenic transcripts and bone mineralization phenotypic markers when the ASCs were grown on FA surfaces compared to metal surfaces at 7 and 21 days. In addition to BMP and TGFβ signaling pathways, EGF and FGF pathways also appeared to be involved in ASC differentiation and mineralization. In vivo studies showed accelerated and enhanced mineralized tissue formation integrated within ordered FA coatings. After 5 weeks, over 80 % of the ordered FA coating was integrated with a mineralized tissue layer covering the implants. Both the intrinsic properties of the FA crystals and the topography of the FA coating appeared to dominate the cell differentiation and mineralization process.
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影响因子：
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