Baculoviral inhibitor of apoptosis protein repeat-containing protein 3 delays early Wallerian degeneration after sciatic nerve injury.
Baculoviral inhibitor of apoptosis protein repeat-containing protein 3 delays early Wallerian degeneration after sciatic nerve injury.
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DOI：
10.4103/1673-5374.322474
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发表时间：
2022-04
影响因子：
6.1
通讯作者：
Yao DB
中科院分区：
文献类型：
--
作者：
Cai M;Shao J;Yung B;Wang Y;Gao NN;Xu X;Zhang HH;Feng YM;Yao DB
沃勒变性是神经损伤后发生的复杂生物学过程，涉及神经变性和再生。雪旺细胞在周围神经系统沃勒变性的细胞和分子事件中起着至关重要的作用。然而，Wallerian变性调节神经损伤和修复的机制仍不清楚，尤其是早期反应。我们以前曾报道过一些关键的调节因子的沃勒变性后坐骨神经损伤。杆状病毒凋亡抑制蛋白重复序列蛋白3（BIRC 3）是调控凋亡抑制蛋白的重要因子。本研究建立了大鼠右侧坐骨神经损伤模型。还建立了体外雪旺细胞模型，并进行基因转染以抑制和过表达BIRC 3。数据表明，BIRC 3表达在坐骨神经损伤后显著上调。BIRC 3的上调和下调均通过激活c-fos和ERK信号通路影响许旺细胞的迁移、增殖和凋亡，并影响相关因子的表达。抑制BIRC 3通过抑制坐骨神经损伤后雪旺细胞的凋亡来延缓早期Wallerian变性。这些发现表明BIRC 3在周围神经损伤修复和再生中起重要作用。本研究于2019年3月1日获得中国南通大学机构动物管理和使用委员会批准（批准号：2019-nsfc 004）。
Wallerian degeneration is a complex biological process that occurs after nerve injury, and involves nerve degeneration and regeneration. Schwann cells play a crucial role in the cellular and molecular events of Wallerian degeneration of the peripheral nervous system. However, Wallerian degeneration regulating nerve injury and repair remains largely unknown, especially the early response. We have previously reported some key regulators of Wallerian degeneration after sciatic nerve injury. Baculoviral inhibitor of apoptosis protein repeat-containing protein 3 (BIRC3) is an important factor that regulates apoptosis-inhibiting protein. In this study, we established rat models of right sciatic nerve injury. In vitro Schwann cell models were also established and subjected to gene transfection to inhibit and overexpress BIRC3. The data indicated that BIRC3 expression was significantly up-regulated after sciatic nerve injury. Both BIRC3 upregulation and downregulation affected the migration, proliferation and apoptosis of Schwan cells and affected the expression of related factors through activating c-fos and ERK signal pathway. Inhibition of BIRC3 delayed early Wallerian degeneration through inhibiting the apoptosis of Schwann cells after sciatic nerve injury. These findings suggest that BIRC3 plays an important role in peripheral nerve injury repair and regeneration. The study was approved by the Institutional Animal Care and Use Committee of Nantong University, China (approval No. 2019-nsfc004) on March 1, 2019.
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DOI：
10.1016/j.cub.2017.01.062
发表时间：
2017-03-20
期刊：
Current biology : CB
影响因子：
--
作者：
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通讯作者：
Deppmann CD
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影响因子：
2.4
作者：
Liu X;Sun Y;Li H;Li Y;Li M;Yuan Y;Cui S;Yao D
通讯作者：
Yao D
影响因子：
3.7
作者：
Hirakawa, H;Okajima, S;Oyamada, M
通讯作者：
Oyamada, M
TGF-β1 对于大鼠坐骨神经损伤后的华勒氏变性至关重要
影响因子：
3.3
作者：
Li, M.;Zhang, P.;Yao, D.
通讯作者：
Yao, D.
影响因子：
3.4
作者：
Adalbert, Robert;Nogradi, Antal;Coleman, Michael P.
通讯作者：
Coleman, Michael P.