Serogroups of Dichelobacter nodosus, the cause of footrot in sheep, are randomly distributed across England.
Serogroups of Dichelobacter nodosus, the cause of footrot in sheep, are randomly distributed across England.
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DOI：
10.1038/s41598-020-73750-5
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发表时间：
2020-10-08
影响因子：
4.6
通讯作者：
Purdy KJ
中科院分区：
文献类型：
--
作者：
Prosser NS;Monaghan EM;Green LE;Purdy KJ
我们提出了英格兰最大、最具代表性的结节双菌血清学多样性研究。 D. nodosus 引起腐蹄病，是全球最重要的五种绵羊疾病之一。商业疫苗含有九个血清群，与二价疫苗相比功效较低。我们的目的是调查英格兰 D. nodosus 血清群的流行率和分布，以阐明二价疫苗是否可以保护全国鸡群。 164 个羊群的农民提交了来自 8 只羊（最好是患病羊）的 8 份指间拭子。所有血清群（A-I）均通过 PCR 在 687/1150 例结节线虫阳性拭子中检测到，每个血清群的阳性拭子患病率为 2.6-69.3%。每个鸡群平均有两个血清群（范围 0-6）。血清群在鸡群之间随机分布，但在鸡群内部聚集，鸡群之间有 50 种血清群组合。 H 和 B 是最流行的血清群，分别存在于 > 60% 的鸡群中，但仅 27% 的鸡群中同时存在。因此，针对这两个血清群的二价疫苗将完全保护 27% 的鸡群（如果仅存在 H 和 B），如果鸡群中存在更多血清群，则可以部分保护。我们得出的结论是，一种二价疫苗无法保护全国鸡群免受腐蹄病的侵害，并且由于鸡群中有 50 种血清群组合，因此需要针对鸡群的疫苗。
We present the largest and most representative study of the serological diversity of Dichelobacter nodosus in England. D. nodosus causes footrot and is one of the top five globally important diseases of sheep. The commercial vaccine, containing nine serogroups, has low efficacy compared with bivalent vaccines. Our aim was to investigate the prevalence and distribution of serogroups of D. nodosus in England to elucidate whether a bivalent vaccine could protect the national flock. Farmers from 164 flocks submitted eight interdigital swabs from eight, preferably diseased, sheep. All serogroups, A–I, were detected by PCR in 687/1150 D. nodosus positive swabs, with a prevalence of 2.6–69.3% of positive swabs per serogroup. There was a median of two serogroups per flock (range 0–6). Serogroups were randomly distributed between, but clustered within, flocks, with 50 combinations of serogroups across flocks. H and B were the most prevalent serogroups, present in > 60% of flocks separately but in only 27% flocks together. Consequently, a bivalent vaccine targeting these two serogroups would protect 27% of flocks fully (if only H and B present) and partially, if more serogroups were present in the flock. We conclude that one bivalent vaccine would not protect the national flock against footrot and, with 50 combinations of serogroups in flocks, flock-specific vaccines are necessary.
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影响因子：
5.5
作者：
HUNT, JD;JACKSON, DC;STEWART, DJ
通讯作者：
STEWART, DJ
影响因子：
4.6
作者：
Humbert, Maria Victoria;Jackson, Alexandra;Christodoulides, Myron
通讯作者：
Christodoulides, Myron
影响因子：
0.8
作者：
EGERTON, JR;ROBERTS, DS;PARSONSON, IM
通讯作者：
PARSONSON, IM
影响因子：
1.1
作者：
Allworth, M. B.;Egerton, J. R.
通讯作者：
Egerton, J. R.
影响因子：
1.1
作者：
CLAXTON, PD;RIBEIRO, LA;EGERTON, JR
通讯作者：
EGERTON, JR