Differential analysis of microbiomes in mucus and tissues obtained from colorectal cancer patients.
Differential analysis of microbiomes in mucus and tissues obtained from colorectal cancer patients.
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DOI：
10.1038/s41598-022-21928-4
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发表时间：
2022-10-28
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
--
作者：
结直肠上皮的外粘液层易于移除并被肠道微生物群定殖，而内粘液层牢固地附着于上皮并且没有细菌。虽然穿透内粘液层的特定细菌可以接触上皮细胞并引发癌症发展，但大多数研究忽略了采样时粘液粘附的程度。因此，我们评估了是否可以通过去除外粘液层来识别粘附在组织上的细菌。我们对18例结直肠癌手术标本的16S rRNA基因测序分析显示，Sutterella（P = 0.045）和Enterobacteriaceae（P = 0.045）在覆盖粘膜的粘液中相对于粘膜显著富集。与相同的癌组织相比，Rikenellaceae（P = 0.026）在覆盖癌组织的粘液中显著富集。与覆盖癌症的粘液相比，覆盖粘膜的粘液中显著富集瘤胃球菌科（P = 0.015）、肠杆菌科（P = 0.030）和丹毒丝菌科（P = 0.028）。与覆盖粘膜的粘液相比，覆盖癌症的粘液中梭杆菌（P = 0.038）显着富集。比较粘液和去除粘液的组织的微生物组可能有助于识别真正侵入组织并影响肿瘤发生的细菌。
The outer mucus layer of the colorectal epithelium is easily removable and colonized by commensal microbiota, while the inner mucus layer is firmly attached to the epithelium and devoid of bacteria. Although the specific bacteria penetrating the inner mucus layer can contact epithelial cells and trigger cancer development, most studies ignore the degree of mucus adhesion at sampling. Therefore, we evaluated whether bacteria adhering to tissues could be identified by removing the outer mucus layer. Our 16S rRNA gene sequencing analysis of 18 surgical specimens of human colorectal cancer revealed that Sutterella (P = 0.045) and Enterobacteriaceae (P = 0.045) were significantly enriched in the mucus covering the mucosa relative to the mucosa. Rikenellaceae (P = 0.026) was significantly enriched in the mucus covering cancer tissues compared with those same cancer tissues. Ruminococcaceae (P = 0.015), Enterobacteriaceae (P = 0.030), and Erysipelotrichaceae (P = 0.028) were significantly enriched in the mucus covering the mucosa compared with the mucus covering cancers. Fusobacterium (P = 0.038) was significantly enriched in the mucus covering cancers compared with the mucus covering the mucosa. Comparing the microbiomes of mucus and tissues with mucus removed may facilitate identifying bacteria that genuinely invade tissues and affect tumorigenesis.
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影响因子：
6
作者：
Okuda S;Shimada Y;Tajima Y;Yuza K;Hirose Y;Ichikawa H;Nagahashi M;Sakata J;Ling Y;Miura N;Sugai M;Watanabe Y;Takeuchi S;Wakai T
通讯作者：
Wakai T
影响因子：
14.9
作者：
Kaminuma E;Mashima J;Kodama Y;Gojobori T;Ogasawara O;Okubo K;Takagi T;Nakamura Y
通讯作者：
Nakamura Y
影响因子：
3.9
作者：
Fukui, Hirokazu;Nishida, Akifumi;Miwa, Hiroto
通讯作者：
Miwa, Hiroto
影响因子：
12.2
作者：
Araujo-Perez, Felix;McCoy, Amber N.;Keku, Temitope O.
通讯作者：
Keku, Temitope O.
DOI：
10.1152/ajpgi.2001.280.5.g922
发表时间：
2001-05-01
影响因子：
4.5
作者：
Atuma, C;Strugala, V;Holm, L
通讯作者：
Holm, L