RNA-Seq Identification of Cd Responsive Transporters Provides Insights into the Association of Oxidation Resistance and Cd Accumulation in Cucumis sativus L.
RNA-Seq Identification of Cd Responsive Transporters Provides Insights into the Association of Oxidation Resistance and Cd Accumulation in Cucumis sativus L.
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Cd 响应转运蛋白的 RNA-Seq 鉴定提供了对黄瓜抗氧化性和 Cd 积累之间关系的见解。
DOI：
10.3390/antiox10121973
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发表时间：
2021-12-10
期刊：
影响因子：
--
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Feng S;Shen Y;Xu H;Dong J;Chen K;Xiang Y;Jiang X;Yao C;Lu T;Huan W;Wang H
温室蔬菜生产快速增长，已成为我国黄瓜生产的主要力量。在高强度的GVP系统中，过量施肥会导致土壤酸化，增加蔬菜中Cd的积累和氧化胁迫损伤，并增加蔬菜消费者的健康风险。因此，提高抗氧化能力和激活Cd转运蛋白基因的表达水平是提高植物对Cd胁迫的抗性和降低Cd累积浓度的可行途径。在这里，我们使用转录组学，以确定五个黄瓜转运蛋白基因（CsNRAMP1，CsNRAMP4，CsHMA1，CsZIP1，CsZIP8）在镉胁迫下，这是参与镉在酵母中的运输活动。离子组学、基因表达和REDOX反应水平关联分析表明，CsNRAMP4的转录与黄瓜根系的Cd积累和抗氧化能力呈正相关。CsHMA1的表达水平与镉诱导的抗氧化能力呈负相关。CsHMA1的过表达显著缓解镉胁迫诱导的抗氧化活性。此外，CsHMA2高表达植株对Cd的生物富集作用显著。嫁接实验证实，CsHMA1与黄瓜的抗氧化能力有关，而CsHMA2则与Cd从根向地上部的转运有关。本研究阐明了园艺甜瓜中Cd转运和氧化损伤清除的一种新的调控机制，为通过调控植物体内Cd积累和抗性来人工调控Cd转运提供了新的思路。
Greenhouse vegetable production (GVP) has grown rapidly and has become a major force for cucumber production in China. In highly intensive GVP systems, excessive fertilization results in soil acidification, increasing Cd accumulation and oxidative stress damage in vegetables as well as increasing health risk of vegetable consumers. Therefore, enhancing antioxidant capacity and activating the expression level of Cd transporter genes seem to be feasible solutions to promote plant resistance to Cd stress and to reduce accumulated Cd concentration. Here, we used transcriptomics to identify five cucumber transporter genes (CsNRAMP1, CsNRAMP4, CsHMA1, CsZIP1, and CsZIP8) in response to cadmium stress, which were involved in Cd transport activity in yeast. Ionomics, gene expression, and REDOX reaction level association analyses have shown that the transcript of CsNRAMP4 was positively correlated with Cd accumulation and antioxidant capacity of cucumber roots. The expression level of CsHMA1 was negatively correlated with Cd-induced antioxidant capacity. The overexpression of CsHMA1 significantly relieved Cd stress-induced antioxidant activities. In addition, shoots with high CsHMA2 expression remarkably presented Cd bioaccumulation. Grafting experiments confirmed that CsHMA1 contributed to the high antioxidant capacity of cucumber, while CsHMA2 was responsible for the transport of Cd from the roots to the shoots. Our study elucidated a novel regulatory mechanism for Cd transport and oxidative damage removal in horticultural melons and provided a perspective to regulate Cd transport artificially by modulating Cd accumulation and resistance in plants.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
7.4
作者：
Lee, Sichul;Kim, Yu-Young;An, Gynheung
通讯作者：
An, Gynheung
MicroRNA166 调节水稻的镉耐受性和积累
影响因子：
7.4
作者：
Ding, Yanfei;Gong, Shaohua;Zhu, Cheng
通讯作者：
Zhu, Cheng
降低水稻镉浓度的新型金属伴侣的功能表征
影响因子：
11.1
作者：
Khan, Irfan Ullah;Rono, Justice Kipkorir;Yang, Zhi Min
通讯作者：
Yang, Zhi Min
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro