Static Magnetic Fields Reduce Oxidative Stress to Improve Wound Healing and Alleviate Diabetic Complications.
Static Magnetic Fields Reduce Oxidative Stress to Improve Wound Healing and Alleviate Diabetic Complications.
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静磁场可减少氧化应激，促进伤口愈合并减轻糖尿病并发症。
DOI：
10.3390/cells11030443
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发表时间：
2022-01-27
期刊：
影响因子：
6
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Feng C;Yu B;Song C;Wang J;Zhang L;Ji X;Wang Y;Fang Y;Liao Z;Wei M;Zhang X
尽管一些研究表明某些静磁场（SMFs）能够促进糖尿病小鼠的伤口愈合，但尚不清楚其他糖尿病并发症，如肝脏疾病和糖尿病肾病是否也能得到缓解。在此，我们使用钕永磁体构建了两个简易磁板，以检测适度静磁场对遗传性肥胖的瘦素受体缺陷型db/db糖尿病小鼠的综合影响。我们发现，尽管这两种静磁场设置并未使血糖明显降低，但糖化血清蛋白（GSP）和丙二醛（MDA）水平均显著下降（科恩d值 = 2.57 - 3.04）。此外，静磁场治疗显著改善了伤口愈合、肝脏脂质堆积和肾脏损伤情况（科恩d值 = 0.91 - 2.05）。伤口组织检查显示核因子红细胞2相关因子2（NRF2）水平明显降低（科恩d值 = 2.49 - 5.40），Ki - 67水平升高（科恩d值 = 2.30 - 3.40），这表明氧化应激降低且细胞增殖增加。对成纤维细胞NIH3T3细胞进行的体外细胞研究表明，静磁场能够减少高糖诱导的NRF2核转位（科恩d值 = 0.87 - 1.15）以及细胞活性氧（ROS）升高（科恩d值 = 0.92），这表明氧化应激降低。因此，静磁场治疗改善了高糖诱导的细胞活力、增殖和迁移损伤。所以，我们的研究结果表明，这些简易的静磁场装置能够有效降低糖尿病小鼠的氧化应激，并且可能在未来为缓解多种糖尿病并发症提供一种具有成本效益的物理治疗策略。
Although some studies have shown that some static magnetic fields (SMFs) can promote wound healing in diabetic mice, it is not clear whether the other diabetes complications, such as liver disease and diabetic nephropathy, can also be alleviated. Here, we constructed two simple magnetic plates using neodymium permanent magnets to examine the comprehensive effects of moderate SMFs on genetically obese leptin receptor-deficient db/db diabetic mice. We found that although the blood glucose was not obviously reduced by these two SMF settings, both of the glycated serum protein (GSP) and malondialdehyde (MDA) levels were significantly decreased (Cohen’s d = 2.57–3.04). Moreover, the wound healing, liver lipid accumulation, and renal defects were all significantly improved by SMF treatment (Cohen’s d = 0.91–2.05). Wound tissue examination showed obvious nuclear factor erythroid 2-related factor 2 (NRF2) level decrease (Cohen’s d = 2.49–5.40) and Ki-67 level increase (Cohen’s d = 2.30–3.40), indicating decreased oxidative stress and increased cell proliferation. In vitro cellular studies with fibroblast NIH3T3 cells showed that SMFs could reduce high glucose-induced NRF2 nucleus translocation (Cohen’s d = 0.87–1.15) and cellular reactive oxygen species (ROS) elevation (Cohen’s d = 0.92), indicating decreased oxidative stress. Consequently, high glucose-induced impairments in cell vitality, proliferation, and migration were all improved by SMF treatment. Therefore, our results demonstrate that these simple SMF devices could effectively reduce oxidative stress in diabetic mice and may provide a cost-effective physical therapy strategy to alleviate multiple diabetic complications in the future.
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