Transurethral versus suprapubic catheterization to test urethral function in rats.
Transurethral versus suprapubic catheterization to test urethral function in rats.
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DOI：
10.1038/s41598-021-93772-x
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发表时间：
2021-07-13
影响因子：
4.6
通讯作者：
Damaser MS
中科院分区：
文献类型：
--
作者：
Janssen K;Deng K;Majerus SJA;Lin DL;Hanzlicek B;Butler RS;van der Vaart CH;Damaser MS
经尿道和耻骨上导尿都被用来测试大鼠的尿道功能;然而，尚不清楚这些方法是否会影响尿道功能，或者导尿的顺序是否会影响结果。本交叉设计实验的目的是比较导管插入方法和顺序对漏点压力（LPP）测试的影响。以交叉设计在麻醉的雌性未交配Sprague-Dawley大鼠中记录LPP和同时的外尿道括约肌肌电图（EUS EMG），以测试经尿道和耻骨上导管插入术的效果。在LPP测试期间，无论是用经尿道导管还是耻骨上导管测量，峰值膀胱压力均无显著差异。第一次和第二次导管插入之间的峰值膀胱压力没有显著差异。然而，与第二次插入导管相比，第一次插入导管后的峰值EMG放电率以及峰值EMG振幅和峰值与基线之间的EMG振幅差显著更高，与导管方法无关。我们的研究结果表明，导尿途径不会改变尿道功能，例如，造成功能性部分出口梗阻。两种方法均可用于大鼠LPP和/或EUS EMG测试。
Transurethral and suprapubic catheterization have both been used to test urethral function in rats; however, it is unknown whether these methods affect urethral function or if the order of catheterization affects the results. The aim of this cross-over designed experiment was to compare the effects of catheterization methods and order on leak point pressure (LPP) testing. LPP and simultaneous external urethral sphincter electromyography (EUS EMG) were recorded in anesthetized female virgin Sprague-Dawley rats in a cross-over design to test the effects of transurethral and suprapubic catheterization. There was no significant difference in peak bladder pressure during LPP testing whether measured with a transurethral or suprapubic catheter. There was no significant difference in peak bladder pressure between the first and second catheter insertions. However, peak EMG firing rate, as well as peak EMG amplitude and EMG amplitude difference between peak and baseline were significantly higher after the first catheter insertion compared to the second insertion, regardless of the catheter method. Our results suggest that route of catheterization does not alter urethral function, e.g. create a functional partial outlet obstruction. Either catheterization method could be used for LPP and/or EUS EMG testing in rats.
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